Anti-alpha 1 Glycine Receptor/GLRA1抗体(ab228541)
Key features and details
- Rabbit polyclonal to alpha 1 Glycine Receptor/GLRA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-alpha 1 Glycine Receptor/GLRA1抗体
参阅全部 alpha 1 Glycine Receptor/GLRA1 一抗 -
描述
兔多克隆抗体to alpha 1 Glycine Receptor/GLRA1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Cow, Zebrafish, Rhesus monkey -
免疫原
Recombinant fragment within Human alpha 1 Glycine Receptor/GLRA1 (internal sequence). The exact sequence is proprietary.
Database link: P23415 -
阳性对照
- WB: HeLa S3 whole cell lysate. IHC-P: OVCA xenograft tissue. ICC/IF: DIV9 rat E18 primary cortical neuron cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab228541于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
|
说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing). -
疾病相关
Defects in GLRA1 are a cause of startle disease (STHE) [MIM:149400]; also known as hereditary hyperekplexia or congenital stiff-person syndrome. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli. Inheritance can be autosomal dominant or recessive. -
序列相似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA1 sub-subfamily. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 281783 Cow
- Entrez Gene: 2741 Human
- Entrez Gene: 14654 Mouse
- Entrez Gene: 25674 Rat
- Entrez Gene: 713940 Rhesus monkey
- Entrez Gene: 30676 Zebrafish
- Omim: 138491 Human
- SwissProt: P57695 Cow
see all -
别名
- GLRA1 antibody
- GLRA1_HUMAN antibody
- Glycine receptor 48 kDa subunit antibody
see all
图片
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Paraffin-embedded OVCA (human) xenograft tissue stained for alpha 1 Glycine Receptor/GLRA1 using ab228541 at 1/100 dilution in immunohistochemical analysis.
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4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neuron cells labeling alpha 1 Glycine Receptor/GLRA1 using ab228541 at 1/500 dilution.
The nuclear counterstain is DAPI (blue). beta Tubulin is labeled with an anti-beta Tubulin antibody (red).
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Anti-alpha 1 Glycine Receptor/GLRA1 antibody (ab228541) at 1/1500 dilution + HeLa S3 whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa7.5% SDS-PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab228541 被引用在 1 文献中.
- Chen K et al. Single-cell RNA-seq transcriptomic landscape of human and mouse islets and pathological alterations of diabetes. iScience 25:105366 (2022). PubMed: 36339258