Anti-ALMS1抗体(ab4306)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALMS1 antibody (ab4306)

    Key features and details

    • Goat polyclonal to ALMS1
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-ALMS1抗体
      参阅全部 ALMS1 一抗

    • 描述

      山羊多克隆抗体to ALMS1

    • 宿主

      Goat

    • 特异性

      The C terminus of ALMS 1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis. ALMS1 protein has got a molecular weight of approximately 460kD. ab4306 results in a WB band of the right size, confirming that this antibody recognises the right protein.

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse

    • 免疫原

      Synthetic peptide: RVTNQLLGRKVPWD, corresponding to C terminal amino acids 4145-4167 of Human ALMS1.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      GenBank Accession Number – NP_055935. LocusLink ID - 7840 (human); 236266 (mouse).

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% Tris, 0.5% BSA
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4306于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use a concentration of 1 - 3 µg/ml.
    说明
    IHC-P
    Use a concentration of 1 - 3 µg/ml.

    靶标

    • 功能

      Possible role in intracellular trafficking.

    • 组织特异性

      Expressed in all tissues tested including adipose and pancreas. Expressed by beta-cells of the islets in the pancreas (at protein level).

    • 疾病相关

      Defects in ALMS1 are the cause of Alstrom syndrome (ALMS) [MIM:203800]. Alstrom syndrome is a rare autosomal recessive disorder characterized by progressive cone-rod retinal dystrophy, neurosensory hearing loss, early childhood obesity and type 2 diabetes mellitus. Dilated cardiomyopathy, acanthosis nigricans, male hypogonadism, hypothyroidism, developmental delay and hepatic dysfunction can also be associated with the syndrome.

    • 发展阶段

      Widely expressed in fetal tissues. Detected in fetal pancreas, skeletal muscle, liver, kidney and brain (at protein level). Expressed in fetal aorta and brain.

    • 翻译后修饰

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • 细胞定位

      Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > spindle pole. Associated with centrosomes and basal body at the base of primary cilia. During mitosis localizes to both spindle poles.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Alms1 antibody
        • ALMS1_HUMAN antibody
        • ALSS antibody
        • Alstrom syndrome 1 antibody
        • Alstrom syndrome protein 1 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALMS1 antibody (ab4306)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALMS1 antibody (ab4306)

        Paraffin embedded human testis tissue stained for ALMS1 using ab4306 at 2 µg/ml in immunohistochemical analysis.

      文献 (1)

      发表研究结果有使用 ab4306？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab4306 被引用在 1 文献中.

      • Maddalena A  et al. Triple Vectors Expand AAV Transfer Capacity in the Retina. Mol Ther 26:524-541 (2018). PubMed: 29292161

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      Western blot abreview for Anti-ALMS1 antibody

       Poor
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (kidney)
      Gel Running Conditions
      Reduced Denaturing (6%)
      Loading amount
      100 µg
      Specification
      kidney
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
      Read More

      Ankita

      Verified customer

      提交于 Oct 29 2014

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