Key features and details
- Goat polyclonal to ALMS1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
参阅全部 ALMS1 一抗
特异性The C terminus of ALMS 1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis. ALMS1 protein has got a molecular weight of approximately 460kD. ab4306 results in a WB band of the right size, confirming that this antibody recognises the right protein.
经测试应用适用于: WB, IHC-Pmore details
常规说明GenBank Accession Number – NP_055935. LocusLink ID - 7840 (human); 236266 (mouse).
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab4306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200. Detects a band of approximately 460 kDa (predicted molecular weight: 460 kDa).
A 1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 1 - 3 µg/ml.|
功能Possible role in intracellular trafficking.
组织特异性Expressed in all tissues tested including adipose and pancreas. Expressed by beta-cells of the islets in the pancreas (at protein level).
疾病相关Defects in ALMS1 are the cause of Alstrom syndrome (ALMS) [MIM:203800]. Alstrom syndrome is a rare autosomal recessive disorder characterized by progressive cone-rod retinal dystrophy, neurosensory hearing loss, early childhood obesity and type 2 diabetes mellitus. Dilated cardiomyopathy, acanthosis nigricans, male hypogonadism, hypothyroidism, developmental delay and hepatic dysfunction can also be associated with the syndrome.
发展阶段Widely expressed in fetal tissues. Detected in fetal pancreas, skeletal muscle, liver, kidney and brain (at protein level). Expressed in fetal aorta and brain.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > spindle pole. Associated with centrosomes and basal body at the base of primary cilia. During mitosis localizes to both spindle poles.
- Information by UniProt
- Alms1 antibody
- ALMS1_HUMAN antibody
- ALSS antibody
g of Hela extract were run on a Tris Acetate NuPage 3-8% gel and probed with a 1:200 dilution of the ab4306 antibody. µ
The 460kD band represents ALMS1.
The 460kD band represents ALMS1.
ab4306 (2µg/ml) staining of paraffin embedded Human Testis.
ab4306 被引用在 1 文献中.
- Maddalena A et al. Triple Vectors Expand AAV Transfer Capacity in the Retina. Mol Ther 26:524-541 (2018). PubMed: 29292161