Anti-ALMS1抗体(ab4306)
Key features and details
- Goat polyclonal to ALMS1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ALMS1抗体
参阅全部 ALMS1 一抗 -
描述
山羊多克隆抗体to ALMS1 -
宿主
Goat -
特异性
The C terminus of ALMS 1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis. ALMS1 protein has got a molecular weight of approximately 460kD. ab4306 results in a WB band of the right size, confirming that this antibody recognises the right protein. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide: RVTNQLLGRKVPWD, corresponding to C terminal amino acids 4145-4167 of Human ALMS1.
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常规说明
GenBank Accession Number – NP_055935. LocusLink ID - 7840 (human); 236266 (mouse).
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab4306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/200. Detects a band of approximately 460 kDa (predicted molecular weight: 460 kDa). A 1 hour primary incubation is recommended for this product. |
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| IHC-P | Use a concentration of 1 - 3 µg/ml. |
靶标
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功能
Possible role in intracellular trafficking. -
组织特异性
Expressed in all tissues tested including adipose and pancreas. Expressed by beta-cells of the islets in the pancreas (at protein level). -
疾病相关
Defects in ALMS1 are the cause of Alstrom syndrome (ALMS) [MIM:203800]. Alstrom syndrome is a rare autosomal recessive disorder characterized by progressive cone-rod retinal dystrophy, neurosensory hearing loss, early childhood obesity and type 2 diabetes mellitus. Dilated cardiomyopathy, acanthosis nigricans, male hypogonadism, hypothyroidism, developmental delay and hepatic dysfunction can also be associated with the syndrome. -
发展阶段
Widely expressed in fetal tissues. Detected in fetal pancreas, skeletal muscle, liver, kidney and brain (at protein level). Expressed in fetal aorta and brain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > spindle pole. Associated with centrosomes and basal body at the base of primary cilia. During mitosis localizes to both spindle poles. - Information by UniProt
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数据库链接
- Entrez Gene: 7840 Human
- Entrez Gene: 236266 Mouse
- Omim: 606844 Human
- SwissProt: Q8TCU4 Human
- SwissProt: Q8K4E0 Mouse
- Unigene: 184720 Human
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别名
- Alms1 antibody
- ALMS1_HUMAN antibody
- ALSS antibody
see all
图片
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Western blot - Anti-ALMS1 antibody (ab4306)20
µ The 460kD band represents ALMS1.
20 µg of Hela extract were run on a Tris Acetate NuPage 3-8% gel and probed with a 1:200 dilution of the ab4306 antibody.
The 460kD band represents ALMS1. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALMS1 antibody (ab4306)ab4306 (2µg/ml) staining of paraffin embedded Human Testis.
数据表及文件
文献 (1)
ab4306 被引用在 1 文献中.
- Maddalena A et al. Triple Vectors Expand AAV Transfer Capacity in the Retina. Mol Ther 26:524-541 (2018). PubMed: 29292161
