Anti-ALMS1抗体(ab4306)

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Key features and details

  • Goat polyclonal to ALMS1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-ALMS1抗体
    参阅全部 ALMS1 一抗

  • 描述

    山羊多克隆抗体to ALMS1

  • 宿主

    Goat

  • 特异性

    The C terminus of ALMS 1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis. ALMS1 protein has got a molecular weight of approximately 460kD. ab4306 results in a WB band of the right size, confirming that this antibody recognises the right protein.

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide: RVTNQLLGRKVPWD, corresponding to C terminal amino acids 4145-4167 of Human ALMS1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    GenBank Accession Number – NP_055935. LocusLink ID - 7840 (human); 236266 (mouse).

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris, 0.5% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200. Detects a band of approximately 460 kDa (predicted molecular weight: 460 kDa).

A 1 hour primary incubation is recommended for this product.
IHC-P Use a concentration of 1 - 3 µg/ml.

靶标

  • 功能

    Possible role in intracellular trafficking.

  • 组织特异性

    Expressed in all tissues tested including adipose and pancreas. Expressed by beta-cells of the islets in the pancreas (at protein level).

  • 疾病相关

    Defects in ALMS1 are the cause of Alstrom syndrome (ALMS) [MIM:203800]. Alstrom syndrome is a rare autosomal recessive disorder characterized by progressive cone-rod retinal dystrophy, neurosensory hearing loss, early childhood obesity and type 2 diabetes mellitus. Dilated cardiomyopathy, acanthosis nigricans, male hypogonadism, hypothyroidism, developmental delay and hepatic dysfunction can also be associated with the syndrome.

  • 发展阶段

    Widely expressed in fetal tissues. Detected in fetal pancreas, skeletal muscle, liver, kidney and brain (at protein level). Expressed in fetal aorta and brain.

  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > spindle pole. Associated with centrosomes and basal body at the base of primary cilia. During mitosis localizes to both spindle poles.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Alms1 antibody
      • ALMS1_HUMAN antibody
      • ALSS antibody
      • Alstrom syndrome 1 antibody
      • Alstrom syndrome protein 1 antibody
      see all

    图片

    • Western blot - Anti-ALMS1 antibody (ab4306)
      Western blot - Anti-ALMS1 antibody (ab4306)

      20 µg of Hela extract were run on a Tris Acetate NuPage 3-8% gel and probed with a 1:200 dilution of the ab4306 antibody.

      The 460kD band represents ALMS1.

       

      20 µg of Hela extract were run on a Tris Acetate NuPage 3-8% gel and probed with a 1:200 dilution of the ab4306 antibody.

      The 460kD band represents ALMS1.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALMS1 antibody (ab4306)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALMS1 antibody (ab4306)
      ab4306 (2µg/ml) staining of paraffin embedded Human Testis.

    文献 (1)

    发表研究结果有使用 ab4306？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab4306 被引用在 1 文献中.

    • Maddalena A  et al. Triple Vectors Expand AAV Transfer Capacity in the Retina. Mol Ther 26:524-541 (2018). PubMed: 29292161

    客户评价及客户问答

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    Western blot abreview for Anti-ALMS1 antibody

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (kidney)
    Gel Running Conditions
    Reduced Denaturing (6%)
    Loading amount
    100 µg
    Specification
    kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Read More

    Ankita

    Verified customer

    提交于 Oct 29 2014

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