Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 15µg/mL · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: goat serum + TBS
Secondary antibody
Name
Non-Abcam antibody was used: Vector biotinylated anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/400
Additional data
Additional Notes
Used Vector ABC Elite kit and DAB to amplify and visualize. Little background but positive signal absent beneath developing hair follicles.
Abcam response
Thank you for your review of this previously untested species. We expect mouse reactivity based on homology of the immunogen sequence with mouse AP but affinity of the antibody for this species may be relatively low. Development of an IHC stain will also vary across tissue types.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 11 2012