Alexa Fluor® 647荧光Anti-CNPase抗体[11-5B] (ab201679)
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [11-5B] to CNPase
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 647荧光Anti-CNPase抗体[11-5B]
参阅全部 CNPase 一抗 -
描述
Alexa Fluor® 647荧光小鼠单克隆抗体[11-5B] to CNPase -
宿主
Mouse -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Pig, Rhesus monkey不与反应: Chicken, Guinea pig -
免疫原
Full length native protein (purified) corresponding to Human CNPase.
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阳性对照
- IHC- FFPE sections - Human cerebral cortex (normal)
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常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Batches contain 0.4M arginine. -
Concentration information loading...
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纯度
Affinity purified -
克隆
单克隆 -
克隆编号
11-5B -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab201679于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family. -
细胞定位
Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 280752 Cow
- Entrez Gene: 607694 Dog
- Entrez Gene: 1267 Human
- Entrez Gene: 12799 Mouse
- Entrez Gene: 493772 Pig
- Entrez Gene: 25275 Rat
- Entrez Gene: 641337 Rhesus monkey
- Omim: 123830 Human
see all -
别名
- 2'' antibody
- 2'3' cyclic nucleotide 3' phosphodiesterase antibody
- 3''-cyclic-nucleotide 3''-phosphodiesterase antibody
see all
图片
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IHC image of CNPase staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab201679 at 1/100 (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab201679 尚未被引用在任何文献中。