重组Alexa Fluor® 647荧光Anti-CD161抗体[EPR23831-120] (ab281735)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR23831-120] to CD161
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 647荧光Anti-CD161抗体[EPR23831-120]
参阅全部 CD161 一抗 -
描述
Alexa Fluor® 647荧光兔单克隆抗体[EPR23831-120] to CD161 -
宿主
Rabbit -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: Human peripheral blood mononuclear cell.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23831-120 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281735于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
1/500.
|
说明 |
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Flow Cyt
1/500. |
靶标
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功能
Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. -
组织特异性
Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype. -
序列相似性
Contains 1 C-type lectin domain. -
翻译后修饰
N-glycosylated. Contains sialic acid residues. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3820 Human
- Omim: 602890 Human
- SwissProt: Q12918 Human
- Unigene: 169824 Human
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别名
- C-type lectin domain family 5 member B antibody
- CD161 antibody
- CLEC5B antibody
see all
图片
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Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD161 with ab281735 at 1/500 dilution (0.1µg)(Right) compared with a Rabbit monoclonal isotype control - Alexa Fluor® 647 (Left) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Cells were stained with rabbit IgG or ab281735. Then stained with anti-CD4 conjugated to APC and anti-CD56 conjugated to BV421. Gated on viable CD4(-) population.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab281735 尚未被引用在任何文献中。