Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture media, Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 0.01 mU/well
产品名称Alcohol Dehydrogenase Assay试剂盒
参阅全部 Alcohol Dehydrogenase 试剂盒
样品类型Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell culture media
灵敏度= 0.01 mU/well
Abcam's Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.
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Alcohol dehydrogenase assay protocol summary:
- add samples and standards to wells
- add reaction mix - incubate for 3 min
- analyze with microplate reader, incubate for 30 min to 2 hrs and analyze again
This product is manufactured by BioVision, an Abcam company and was previously called K787 Alcohol Dehydrogenase Activity Colorimetric Assay Kit. K787-100 is the same size as the 100 test size of ab102533.
Alcohol dehydrogenase (Alcohol DH, ADH) (EC 184.108.40.206) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
存放说明Store at -20°C. Please refer to protocols.
组件 标识符 100 tests ADH Assay Buffer WM 1 x 25ml ADH Positive Control (Lyophilized) Green 1 vial Developer (Lyophilized) Red 1 vial Isopropanol Blue 1 x 1ml NADH Standard (Lyophilized) Yellow 1 vial
相关性Alcohol dehydrogenase (Alcohol DH, ADH) (EC 220.127.116.11) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
- ADH alpha subunit
- ADH beta subunit
ab102533 被引用在 3 文献中.
- Abdelwahab EMM et al. Normalization of Enzyme Expression and Activity Regulating Vitamin A Metabolism Increases RAR-Beta Expression and Reduces Cellular Migration and Proliferation in Diseases Caused by Tuberous Sclerosis Gene Mutations. Front Oncol 11:644592 (2021). PubMed: 34178631
- Elshaghabee FM et al. Ethanol Production by Selected Intestinal Microorganisms and Lactic Acid Bacteria Growing under Different Nutritional Conditions. Front Microbiol 7:47 (2016). PubMed: 26858714
- Baxter M et al. Phenotypic and functional analyses show stem cell-derived hepatocyte-like cells better mimic fetal rather than adult hepatocytes. J Hepatol 62:581-9 (2015). PubMed: 25457200