Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (flag-tagged mouse ephrinA5 expressed in CHO cells)
Permeabilization
Yes - 0.1% Triton
Specification
flag-tagged mouse ephrinA5 expressed in CHO cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1% BSA in TBS-0.1% Triton
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-rabbit Alexa594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
We tested a range of anti-ephrinA5 antibodies and this one picked up a lot fewer cells than most others.
positive control: anti-flag immunostaining
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 03 2013