参阅全部 AL-1 一抗
特异性ab70114 detects endogenous levels of total AL-1 protein.
经测试应用适用于: Flow Cyt, IHC-Fr, WB, ELISA, IHC-P, ICC/IFmore details
种属反应性与反应: Mouse, Human
Synthetic peptide corresponding to Human AL-1 (internal sequence).
- A549 cells, HeLa cell extracts and human brain tissue.
Previously labelled as Ephrin A5.
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
纯度Immunogen affinity purified
纯化说明ab70114 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab70114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 22071104
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21628570|
|WB||1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/500 - 1/1000.|
功能May function actively to stimulate axon fasciculation. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase.
序列相似性Belongs to the ephrin family.
细胞定位Cell membrane. Membrane > caveola. Compartmentalized in discrete caveolae-like membrane microdomains.
- Information by UniProt
- EPLG7 antibody
- AF1 antibody
- AL 1 antibody
ab70114 staining anti-AL-1 in wild-type and knockout mouse inferior colliculus/cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation with citric acid. Samples were incubated with primary antibody (1/200 in 1 % TBS/BSA buffer) for 2 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
ab70114 at 1/500 dilution staining AL-1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
ab70114 at 1/50 dilution staining AL-1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide
All lanes : Anti-AL-1 antibody (ab70114) at 1/500 dilution
Lane 1 : HeLa cell extracts
Lane 2 : HeLa cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Shen Q et al. Induction of innervation by encapsulated adipocytes with engineered vitamin A metabolism. Transl Res 192:1-14 (2018). WB . Read more (PubMed: 29144959) »
- Liu YH et al. Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon. Sci Rep 7:42895 (2017). Read more (PubMed: 28276447) »