概述

  • 产品名称
  • 描述
    兔多克隆抗体to AL-1
  • 宿主
    Rabbit
  • 特异性
    ab70114 detects endogenous levels of total AL-1 protein.
  • 经测试应用
    适用于: Flow Cyt, IHC-Fr, WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Human AL-1 (internal sequence).

  • 阳性对照
    • A549 cells, HeLa cell extracts and human brain tissue.
  • 常规说明

    Previously labelled as Ephrin A5.

性能

应用

Our Abpromise guarantee covers the use of ab70114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 22071104

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration. PubMed: 21628570
WB 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

图片

  • ab70114 staining anti-AL-1 in wild-type and knockout mouse inferior colliculus/cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation with citric acid. Samples were incubated with primary antibody (1/200 in 1 % TBS/BSA buffer) for 2 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab70114 at 1/500 dilution staining AL-1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

  • ab70114 at 1/50 dilution staining AL-1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide

  • All lanes : Anti-AL-1 antibody (ab70114) at 1/500 dilution

    Lane 1 : HeLa cell extracts
    Lane 2 : HeLa cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?

文献

This product has been referenced in:
  • Shen Q  et al. Induction of innervation by encapsulated adipocytes with engineered vitamin A metabolism. Transl Res 192:1-14 (2018). WB . Read more (PubMed: 29144959) »
  • Liu YH  et al. Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon. Sci Rep 7:42895 (2017). Read more (PubMed: 28276447) »
See all 7 Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews

Application
Western blot
Sample
Syrian hamster Tissue lysate - whole (Brain tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Brain tissue
Blocking step
Animal Free Blocker as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 23°C

Dr. Sarah Pallas

Verified customer

提交于 Jan 29 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (inferior colliculus/cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization
No
Specification
inferior colliculus/cerebellum
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Jun 04 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brainstem)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brainstem
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

提交于 Apr 05 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Cerebellum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

提交于 Mar 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (flag-tagged mouse ephrinA5 expressed in CHO cells)
Permeabilization
Yes - 0.1% Triton
Specification
flag-tagged mouse ephrinA5 expressed in CHO cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Jan 03 2013

Application
Flow Cytometry
Sample
Human Cell (many normal and tumor cell lines)
Permeabilization
No
Specification
many normal and tumor cell lines

Abcam user community

Verified customer

提交于 Jul 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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