Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AKT1 antibody [Y89]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bile duct ligation in Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC in a PT link
Permeabilization
No
Specification
Bile duct ligation in Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: dako diluent
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: Envision anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

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提交于 Sep 24 2015

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