Anti-AKR7A2抗体(ab97458)
Key features and details
- Rabbit polyclonal to AKR7A2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-AKR7A2抗体
参阅全部 AKR7A2 一抗 -
描述
兔多克隆抗体to AKR7A2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 84 - 357 of Human AKR7A2 (NP_003680).
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阳性对照
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates IF: HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97458于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
IHC-P
1/100 - 1/1000. |
靶标
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功能
Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. -
组织特异性
Detected in brain, liver, small intestine and testis, and at lower levels in heart, prostate, skeletal muscle and spleen. Detected in kidney proximal and distal tubules, endothelial cells lining the Bowman's capsules and some cysts. Detected at low levels in lung and pancreas (at protein level). Widely expressed. -
序列相似性
Belongs to the aldo/keto reductase 2 family. -
细胞定位
Golgi apparatus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 511996 Cow
- Entrez Gene: 8574 Human
- Entrez Gene: 110198 Mouse
- Omim: 603418 Human
- SwissProt: O43488 Human
- SwissProt: Q8CG76 Mouse
- Unigene: 571886 Human
- Unigene: 482154 Mouse
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别名
- AFAR antibody
- AFAR1 antibody
- AFB1 aldehyde reductase 1 antibody
see all
图片
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Anti-AKR7A2 antibody (ab97458) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE -
ab97458, at a 1/200 dilution, staining AKR7A2 in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
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Immunohistochemical analysis of PFA fixed, paraffin-embedded mouse fore brain tissue labeling AKR7A2 with a97458 at 1/500 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab97458 被引用在 1 文献中.
- Mishra P et al. ADHFE1 is a breast cancer oncogene and induces metabolic reprogramming. J Clin Invest 128:323-340 (2018). PubMed: 29202474