Application
Western blot
Sample
Human Tissue lysate - whole (endometrial cancer and adjacent control endometriu)
Loading amount
30 µg
Specification
endometrial cancer and adjacent control endometriu
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/5000
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk in T-TBS
Secondary antibody
Name
Non-Abcam antibody was used: peroxidase-conjugated IgG + IgM Jackson Immunorese
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 36 kDa
Positive control
placenta tissue lysate and Ishikawa cell lysate
Additional data
PubMed ID
23146748: View
Additional Notes
Ish...Ishikawa cell line EC...endometrial cancer tissue AC...adjacent control endometrium P...placenta
We also tested these antibodies by dot blot with the AKR1B10 and AKR1B15 recombinant proteins, and we confirmed that they detect only AKR1B10.
We also tested these antibodies by dot blot with the AKR1B10 and AKR1B15 recombinant proteins, and we confirmed that they detect only AKR1B10.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 12 2013