Anti-AKR1B10抗体(ab96417)
Key features and details
- Rabbit polyclonal to AKR1B10
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-AKR1B10抗体
参阅全部 AKR1B10 一抗 -
描述
兔多克隆抗体to AKR1B10 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human AKR1B10 aa 1-286.
Database link: O60218 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96417于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/10000. Predicted molecular weight: 36 kDa.
|
| ICC/IF |
1/100 - 1/1000.
|
| 说明 |
|---|
|
WB
1/500 - 1/10000. Predicted molecular weight: 36 kDa. |
|
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs. -
组织特异性
Found in many tissues. Highly expressed in small intestine, colon and adrenal gland. -
序列相似性
Belongs to the aldo/keto reductase family. -
细胞定位
Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 57016 Human
- Entrez Gene: 67861 Mouse
- Entrez Gene: 296972 Rat
- Omim: 604707 Human
- SwissProt: O60218 Human
- Unigene: 116724 Human
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别名
- AK1BA_HUMAN antibody
- AKR1B10 antibody
- AKR1B11 antibody
see all
图片
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Western blot - Anti-AKR1B10 antibody (ab96417)Anti-AKR1B10 antibody (ab96417) at 1/5000 dilution + Mouse colon lysate at 50 µg
Predicted band size: 36 kDa -
Western blot - Anti-AKR1B10 antibody (ab96417)All lanes : Anti-AKR1B10 antibody (ab96417) at 1/2500 dilution
Lane 1 : Jurkat cells
Lane 2 : Raji cells
Lane 3 : NCI-H929 cells
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa -
Western blot - Anti-AKR1B10 antibody (ab96417)Anti-AKR1B10 antibody (ab96417) at 1/50000 dilution + Rat colon lysate at 50 µg
Predicted band size: 36 kDa -
Western blot - Anti-AKR1B10 antibody (ab96417)All lanes : Anti-AKR1B10 antibody (ab96417) at 1/1000 dilution
Lane 1 : MOLT-4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-AKR1B10 antibody (ab96417)ab96417, at 1/200 dilution, staining AKR1B10 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab96417 被引用在 4 文献中.
- Tatsuta T et al. Transcriptomic alterations in malignant pleural mesothelioma cells in response to long-term treatment with bullfrog sialic acid-binding lectin. Mol Med Rep 23:N/A (2021). PubMed: 33880588
- Ghosh A et al. Chronic E-Cigarette Exposure Alters the Human Bronchial Epithelial Proteome. Am J Respir Crit Care Med 198:67-76 (2018). WB . PubMed: 29481290
- Huppke P et al. Activating de novo mutations in NFE2L2 encoding NRF2 cause a multisystem disorder. Nat Commun 8:818 (2017). WB ; Human . PubMed: 29018201
- Hevir N et al. Decreased levels of AKR1B1 and AKR1B10 in cancerous endometrium compared to adjacent non-cancerous tissue. Chem Biol Interact : (2012). WB, IHC-P ; Human . PubMed: 23146748