Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
100 U/ml IL-1
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Sheep anti-Rabbit IgG (H+L) Secondary Antibody
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 67 kDa
Additional data
Additional Notes
MEF cells were treated with 100 U/ml IL-1 (Left Lane) and vehicle (Right Lane) overnight. Whole cell lysates were extracted and subject to immunoblot with AIF after blocking with 5% milk at room temperature for 1 hour.
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提交于 Jun 04 2021