Key features and details
- Rabbit polyclonal to AICDA - ChIP Grade
- Suitable for: WB, ChIP
- Reacts with: Human
- Isotype: IgG
产品名称Anti-AICDA抗体- ChIP Grade
参阅全部 AICDA 一抗
描述兔多克隆抗体to AICDA - ChIP Grade
经测试应用适用于: WB, ChIPmore details
Synthetic peptide corresponding to Human AICDA aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in AICDA 293T Cell Transient Overexpression Lysate.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab5197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human AICDA peptide (ab13718).|
|ChIP||Use at an assay dependent concentration.|
功能RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
组织特异性Strongly expressed in lymph nodes and tonsils.
疾病相关Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
序列相似性Belongs to the cytidine and deoxycytidylate deaminase family.
- Information by UniProt
- Activation induced cytidine deaminase antibody
- Activation induced deaminase antibody
- Activation-induced cytidine deaminase antibody
Anti-AICDA antibody - ChIP Grade (ab5197) at 1 µg/ml + AICDA 293T Cell Transient Overexpression Lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
ab5197 被引用在 2 文献中.
- Dayal S et al. High resolution analysis of the chromatin landscape of the IgE switch region in human B cells. PLoS One 6:e24571 (2011). ChIP ; Human . PubMed: 21949728
- Duquette ML et al. AID binds to transcription-induced structures in c-MYC that map to regions associated with translocation and hypermutation. Oncogene 24:5791-8 (2005). PubMed: 15940261