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Immunology Immunoglobulins Class Switching

Anti-AICDA抗体- ChIP Grade (ab5197)

  • Datasheet
Reviews (4)Q&A (4)References (2)

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Western blot - Anti-AICDA antibody - ChIP Grade (ab5197)

    Key features and details

    • Rabbit polyclonal to AICDA - ChIP Grade
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-AICDA antibody [EPR23436-45] - ChIP Grade (ab269454)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-AICDA抗体- ChIP Grade
      参阅全部 AICDA 一抗
    • 描述

      兔多克隆抗体to AICDA - ChIP Grade
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse
    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
      (Peptide available as ab13718)

    • 阳性对照

      • This antibody gave a positive signal in AICDA 293T Cell Transient Overexpression Lysate.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Immunology
      • Immunoglobulins
      • Class Switching
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Homologous Recomb.
      • Epigenetics and Nuclear Signaling
      • ChIP assays
      • ChIP antibodies

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • Human AICDA peptide (ab13718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human AICDA protein (denatured) (ab139251)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab5197于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (2)
    Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human AICDA peptide (ab13718).
    说明
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human AICDA peptide (ab13718).

    靶标

    • 功能

      RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
    • 组织特异性

      Strongly expressed in lymph nodes and tonsils.
    • 疾病相关

      Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
    • 序列相似性

      Belongs to the cytidine and deoxycytidylate deaminase family.
    • Target information above from: UniProt accession Q9GZX7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 57379 Human
      • Entrez Gene: 11628 Mouse
      • Omim: 605257 Human
      • SwissProt: Q9GZX7 Human
      • SwissProt: Q9WVE0 Mouse
      • Unigene: 149342 Human
      • Unigene: 391503 Mouse
      • 别名

        • Activation induced cytidine deaminase antibody
        • Activation induced deaminase antibody
        • Activation-induced cytidine deaminase antibody
        • AICDA antibody
        • AICDA_HUMAN antibody
        • AID antibody
        • ARP 2 antibody
        • ARP2 antibody
        • CDA 2 antibody
        • CDA2 antibody
        • Cytidine aminohydrolase antibody
        • HIGM2 antibody
        • Integrated into Burkitt's lymphoma cell line Ramos antibody
        see all

      图片

      • Western blot - Anti-AICDA antibody - ChIP Grade (ab5197)
        Western blot - Anti-AICDA antibody - ChIP Grade (ab5197)
        Anti-AICDA antibody - ChIP Grade (ab5197) at 1 µg/ml + AICDA 293T Cell Transient Overexpression Lysate at 10 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

        Predicted band size: 24 kDa
        Observed band size: 24 kDa

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (2)

      发表研究结果有使用 ab5197?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab5197 被引用在 2 文献中.

      • Dayal S  et al. High resolution analysis of the chromatin landscape of the IgE switch region in human B cells. PLoS One 6:e24571 (2011). ChIP ; Human . PubMed: 21949728
      • Duquette ML  et al. AID binds to transcription-induced structures in c-MYC that map to regions associated with translocation and hypermutation. Oncogene 24:5791-8 (2005). PubMed: 15940261

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-8 of 8 Abreviews or Q&A

      ChIP abreview for Anti-AICDA antibody - ChIP Grade

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      ChIP
      Sample
      Mouse Cell lysate - whole cell (CH12-F3 cell line)
      Negative control
      CH12-F3 cells AID knock-out
      Specification
      CH12-F3 cell line
      Detection step
      Real-time PCR
      Type
      Cross-linking (X-ChIP)
      Duration of cross-linking step: 8 minute(s) and 0 second(s)
      Specification of the cross-linking agent: Formaldehyde (Fisher #BP531-50
      Positive control
      CH12F3 Cells stimulated with cytokine cocktail (also known as CIT) to induce AID expression. Cocktail composition : + Recombinant human TGFbeta1 (R&D systems #240-B) 1 ng/mL + Recombinant mouse IL-4 (Preprotech #214-14) 10 ng/mL + Functional Grade purified a-mouse CD40 (eBiosciences #16-0401) 1 µg/mL
      Read More

      Noé Seija

      Verified customer

      提交于 Jan 15 2021

      Western blot abreview for Anti-AICDA antibody - ChIP Grade

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (CH12-F3 cell line)
      Gel Running Conditions
      Reduced Denaturing (12)
      Loading amount
      40 µg
      Treatment
      One sample was treated with cytokine cocktail CIT to induce AID expression (see additional notes for more information).
      Specification
      CH12-F3 cell line
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Noé Seija

      Verified customer

      提交于 Jan 15 2021

      Western blot abreview for Anti-AICDA antibody - ChIP Grade

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (AID cDNA expression in 293T cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      30 µg
      Specification
      AID cDNA expression in 293T cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      提交于 Oct 29 2019

      ChIP abreview for Anti-AICDA antibody

      Good
      Abreviews
      Abreviews
      Application
      ChIP
      Sample
      Human Cell lysate - other (Primary tonsillar B cells)
      Specification
      Primary tonsillar B cells
      Type
      Cross-linking (X-ChIP)
      Duration of cross-linking step: 4 minute(s) and 0 second(s)
      Specification of the cross-linking agent: 1% Formaldehyde
      Detection step
      Taqman
      Positive control
      AID ChIP followed by qPCR analysis for known AID binding sites:
      IgG switch region
      IgM switch region
      BIRC3 promoter
      Negative control
      AID ChIP followed by qPCR analysis for known sites where AID is known not to bind:
      Neuro D1 gene
      Read More

      DR. David Fear

      Verified customer

      提交于 Aug 13 2012

      Question

      Would like to try this antibody in flow cytometry.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 12 2012

      Answer

      DISCOUNT CODE: ***
      Expiration date: February 13, 2013
      Value: $337

      This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ab5197 in flow cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

      The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

      Read More

      Abcam Scientific Support

      回复于 Oct 12 2012

      Question

      Phone call requesting further information on Anti-AICDA antibody (ab5197)

      Read More

      Abcam community

      Verified customer

      Asked on Apr 17 2012

      Answer

      Thank you for contacting us this morning.

      As discussed over the phone, I have generated a testing discount for anti-AICDA antibody (ab5197) for use in ChIP:

      Discount code:

      Expiration date:



      This code will give you: 1 free primary antibody of your choice before the expiration date. To redeem this offer, please submit an Abreview forChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site:

      https://www.abcam.com/Abreviews

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

      1) Call to place your order and mention the code to our customer service department;

      2) Include the code in your fax order;

      3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here:

      https://www.abcam.com/collaborationdiscount

      Read More

      Abcam Scientific Support

      回复于 Apr 17 2012

      Question

      Customer called. Indeed customer had used this ab5197 a lot previously. Then as it became unavailable, had to switch to ab59361. However he would like to switch back to ab5197, as the results were somewhat better.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 17 2012

      Answer

      Thank you for the phone call and your patience.

      The laboratory has now provided the images of the results with the new and old batch. The old batch has been tested by a collaborator and the new batch has been tested in the laboratory here - this might explain also variation in the migration pattern.Indeed the new batch looks comparable if slightly better. Please see the attached data. The new batch is the batch currently in stock.

      I hope you find this information useful. Please do not hesitate to contact us again should you have any additional question or concern.

      Read More

      Abcam Scientific Support

      回复于 Apr 17 2012

      Question

      I am a scientist in GSF in Germany. I want to use your company antibodies :AID antibody (ab5197) and UNG antibody (ab13668) in western blot. But my proteins come from chicken B cells. I wonder if I can use your antibodies. Attachment is my protein amino acid sequences. Thank you. I am looking forward to getting your response. Best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Jan 17 2005

      Answer

      Thank you for your enquiry regarding ab5197 and ab13668. Having done a BLAST search (for gallus gallus) for the immunogen sequences used to generate those antibodies I found: 92% homology for AID : Query: 1 NVRWAKGRRETYLC 14 N+RWAKGRRETYLC Sbjct: 17 NLRWAKGRRETYLC 30 88% homology for UNG: Query: 1 CRHFSKTNELLQKSGKKP 18 CRHFSKTNELL+KSGK+P Sbjct: 276 CRHFSKTNELLKKSGKEP 293 We have not tested these antibodies in chicken but should you choose to test them on your chicken B cells please let us know by sending us a review and we will reward you with 50 points on the Abcam Loyalty Scheme which can be redeemed on a number of vouchers. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

      Read More

      Abcam Scientific Support

      回复于 Jan 17 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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