Anti-AGO3抗体(ab3593)
Key features and details
- Rabbit polyclonal to AGO3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-AGO3抗体
参阅全部 AGO3 一抗 -
描述
兔多克隆抗体to AGO3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Human
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免疫原
Synthetic peptide corresponding to Human AGO3 aa 350-450.
(Peptide available asab24437) -
常规说明
Protein: NP_079128 (human isoform a); NP_803171 (human isoform b); NP_700451 (mouse).This product was previously labelled as Argonaute 3 / eIF2C 3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab3593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). | |
| ICC/IF | 1/200. |
靶标
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功能
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. -
序列相似性
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
细胞定位
Cytoplasm > P-body. - Information by UniProt
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数据库链接
- Entrez Gene: 192669 Human
- Entrez Gene: 214150 Mouse
- Entrez Gene: 313593 Rat
- Omim: 607355 Human
- SwissProt: Q9H9G7 Human
- SwissProt: Q8CJF9 Mouse
- Unigene: 657659 Human
- Unigene: 440915 Mouse
see all -
别名
- 5730550L01Rik antibody
- Ago 3 antibody
- Ago3 antibody
see all
图片
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Western blot - Anti-AGO3 antibody (ab3593)All lanes : Anti-AGO3 antibody (ab3593) at 2 µg/ml
Lane 1 : Dorsal Root Ganglia (Rat) Tissue Lysate
Lane 2 : Dorsal Root Ganglia (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-AGO3 antibody (ab3593)Rat dorsal root ganglia were fixed in paraformaldehyde, blocked with 2% BSA for 30 minutes then incubated with ab3593 (1/200 dilution). For further details please refer to Hengst U et al (2006).
实验方案
数据表及文件
文献 (1)
ab3593 被引用在 1 文献中.
- Hengst U et al. Functional and selective RNA interference in developing axons and growth cones. J Neurosci 26:5727-32 (2006). PubMed: 16723529
