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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing RNAi

Anti-AGO3抗体(ab3593)

  • Datasheet
Reviews (5) Submit a question References (1)

Product price, shipping and contact information

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Western blot - Anti-AGO3 antibody (ab3593)
  • Immunocytochemistry/ Immunofluorescence - Anti-AGO3 antibody (ab3593)

Key features and details

  • Rabbit polyclonal to AGO3
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG

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概述

  • 产品名称

    Anti-AGO3抗体
    参阅全部 AGO3 一抗
  • 描述

    兔多克隆抗体to AGO3
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide corresponding to Human AGO3 aa 350-450.
    (Peptide available as ab24437)

  • 常规说明

    Protein: NP_079128 (human isoform a); NP_803171 (human isoform b); NP_700451 (mouse).

     This product was previously labelled as Argonaute 3 / eIF2C 3

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Argonaute and Piwi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human AGO3 protein (ab131912)

应用

Our Abpromise guarantee covers the use of ab3593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ICC/IF 1/200.

靶标

  • 功能

    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.
  • 序列相似性

    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 细胞定位

    Cytoplasm > P-body.
  • Target information above from: UniProt accession Q9H9G7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 192669 Human
    • Entrez Gene: 214150 Mouse
    • Entrez Gene: 313593 Rat
    • Omim: 607355 Human
    • SwissProt: Q9H9G7 Human
    • SwissProt: Q8CJF9 Mouse
    • Unigene: 657659 Human
    • Unigene: 440915 Mouse
    • Unigene: 482388 Mouse
    • Unigene: 483093 Mouse
    • Unigene: 92025 Rat
    see all
  • 别名

    • 5730550L01Rik antibody
    • Ago 3 antibody
    • Ago3 antibody
    • AGO3_HUMAN antibody
    • argonaute 3 antibody
    • Argonaute3 antibody
    • eIF 2C 3 antibody
    • eIF-2C 3 antibody
    • eIF2C 3 antibody
    • Eif2c3 antibody
    • EIF2C3 protein antibody
    • Eukaryotic translation initiation factor 2C 3 antibody
    • Eukaryotic translation initiation factor 2C3 antibody
    • FLJ12765 antibody
    • hAgo3 antibody
    • MGC86946 antibody
    • Protein argonaute-3 antibody
    see all

图片

  • Western blot - Anti-AGO3 antibody (ab3593)
    Western blot - Anti-AGO3 antibody (ab3593)
    All lanes : Anti-AGO3 antibody (ab3593) at 2 µg/ml

    Lane 1 : Dorsal Root Ganglia (Rat) Tissue Lysate
    Lane 2 : Dorsal Root Ganglia (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 97 kDa
    Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-AGO3 antibody (ab3593)
    Immunocytochemistry/ Immunofluorescence - Anti-AGO3 antibody (ab3593)

    Rat dorsal root ganglia were fixed in paraformaldehyde, blocked with 2% BSA for 30 minutes then incubated with ab3593 (1/200 dilution). For further details please refer to Hengst U et al  (2006).

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (1)

    发表研究结果有使用 ab3593?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3593 被引用在 1 文献中.

    • Hengst U  et al. Functional and selective RNA interference in developing axons and growth cones. J Neurosci 26:5727-32 (2006). PubMed: 16723529

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-5 of 5 Abreviews or Q&A

    CLIP abreview for Anti-Argonaute 3 / eIF2C 3 antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    CLIP
    Sample
    Human Cell lysate - whole cell (HEK 293T)
    Specification
    HEK 293T
    Type
    iCLIP
    Irradiation Energy Level (mJ/cm2): 150
    Wavelength (nm): 254
    Positive control
    Anti-hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
    Negative control
    Protein A dynabeads without antibody bound
    Immuno-precipitation step
    Other - Protein A dynabeads
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 21 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Argonaute 3 / eIF2C 3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (testis)
    Specification
    testis
    Fixative
    Formaldehyde
    Antigen retrieval step
    Enzymatic
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Dr. Lynn Dong

    Verified customer

    提交于 Feb 28 2012

    Western blot abreview for Anti-Argonaute 3 / eIF2C 3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human umbilical vein endothelial cells)
    Loading amount
    50 µg
    Specification
    human umbilical vein endothelial cells
    Gel Running Conditions
    Reduced Denaturing (SDS-PAGE 8%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 31 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Argonaute 3 / eIF2C 3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (dissociated dorsal root ganglia)
    Specification
    dissociated dorsal root ganglia
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2%
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 07 2006

    Western blot abreview for Anti-Argonaute 3 / eIF2C 3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (dorsal root ganglia explants)
    Loading amount
    50 µg
    Specification
    dorsal root ganglia explants
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 28 2006

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