Key features and details
- Rabbit polyclonal to AGO3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
参阅全部 AGO3 一抗
经测试应用适用于: WB, ICC/IFmore details
种属反应性与反应: Mouse, Rat
Synthetic peptide corresponding to Human AGO3 aa 350-450.
(Peptide available as
常规说明Protein: NP_079128 (human isoform a); NP_803171 (human isoform b); NP_700451 (mouse).
This product was previously labelled as Argonaute 3 / eIF2C 3
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab3593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).|
功能Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.
序列相似性Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
细胞定位Cytoplasm > P-body.
- Information by UniProt
- 5730550L01Rik antibody
- Ago 3 antibody
- Ago3 antibody
All lanes : Anti-AGO3 antibody (ab3593) at 2 µg/ml
Lane 1 : Dorsal Root Ganglia (Rat) Tissue Lysate
Lane 2 : Dorsal Root Ganglia (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Rat dorsal root ganglia were fixed in paraformaldehyde, blocked with 2% BSA for 30 minutes then incubated with ab3593 (1/200 dilution). For further details please refer to Hengst U et al (2006).
ab3593 被引用在 1 文献中.
- Hengst U et al. Functional and selective RNA interference in developing axons and growth cones. J Neurosci 26:5727-32 (2006). PubMed: 16723529