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Anti-Ago1抗体(ab5070)

  • Datasheet
Reviews (5)Q&A (2)References (38)

Product price, shipping and contact information

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Western blot - Anti-Ago1 antibody (ab5070)
  • Western blot - Anti-Ago1 antibody (ab5070)

Key features and details

  • Rabbit polyclonal to Ago1
  • Suitable for: WB
  • Reacts with: Drosophila melanogaster
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Ago1抗体
    参阅全部 Ago1 一抗
  • 描述

    兔多克隆抗体to Ago1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Drosophila melanogaster
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Drosophila melanogaster Ago1 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab23538)

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Argonaute and Piwi
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Ago1 protein (ab162000)

应用

Our Abpromise guarantee covers the use of ab5070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa.

靶标

  • 功能

    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
  • 序列相似性

    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 细胞定位

    Cytoplasm > P-body.
  • Target information above from: UniProt accession Q9UL18 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 36544 Drosophila melanogaster
    • Entrez Gene: 236511 Mouse
    • SwissProt: Q8CJG1 Mouse
    • Unigene: 7398 Drosophila melanogaster
    • Unigene: 30800 Mouse
    • 别名

      • AGO1 antibody
      • AGO1_HUMAN antibody
      • Argonaute 1 antibody
      • Argonaute 1 RISC catalytic component antibody
      • Argonaute1 antibody
      • eIF 2C 1 antibody
      • eIF-2C 1 antibody
      • eIF2C 1 antibody
      • EIF2C antibody
      • Eif2c1 antibody
      • Eukaryotic translation initiation factor 2C 1 antibody
      • GERP95 antibody
      • Golgi Endoplasmic Reticulum protein 95 kDa antibody
      • hAgo1 antibody
      • Protein argonaute 1 antibody
      • Protein argonaute-1 antibody
      • Putative RNA binding protein Q99 antibody
      • Putative RNA-binding protein Q99 antibody
      • Q99 antibody
      see all

    图片

    • Western blot - Anti-Ago1 antibody (ab5070)
      Western blot - Anti-Ago1 antibody (ab5070)
      Anti-Ago1 antibody (ab5070) at 1 µg/ml + Drosophila Melanogaster embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 97 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds
    • Western blot - Anti-Ago1 antibody (ab5070)
      Western blot - Anti-Ago1 antibody (ab5070)This image is courtesy of an anonymous Abreview
      Anti-Ago1 antibody (ab5070) + Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg

      Secondary
      Sheep anti-rabbit HRP conjugated IgG Polyclonal

      Performed under reducing conditions.

      Predicted band size: 97 kDa



      Blot was blocked with 5% Milk for 1 hour at room temperature.

      See Abreview

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (38)

    发表研究结果有使用 ab5070?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5070 被引用在 38 文献中.

    • Adiliaghdam F  et al. A Requirement for Argonaute 4 in Mammalian Antiviral Defense. Cell Rep 30:1690-1701.e4 (2020). PubMed: 32049003
    • Goh E & Okamura K Hidden sequence specificity in loading of single-stranded RNAs onto Drosophila Argonautes. Nucleic Acids Res 47:3101-3116 (2019). PubMed: 30590701
    • Wessels HH  et al. Global identification of functional microRNA-mRNA interactions in Drosophila. Nat Commun 10:1626 (2019). PubMed: 30967537
    • Mondal T  et al. Two way controls of apoptotic regulators consign DmArgonaute-1 a better clasp on it. PLoS One 13:e0190548 (2018). PubMed: 29385168
    • Zhou L  et al. Importance of miRNA stability and alternative primary miRNA isoforms in gene regulation during Drosophila development. Elife 7:N/A (2018). PubMed: 30024380
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-Ago1 antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549 cells)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    300000 cells
    Specification
    A549 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 13 2016

    Immunoprecipitation abreview for Anti-Ago1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (embryonic cells)
    Total protein in input
    1e+006 cells
    Specification
    embryonic cells
    Immuno-precipitation step
    Protein A
    Read More

    Abcam user community

    Verified customer

    提交于 May 12 2012

    Western blot abreview for Anti-Ago1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (many whole cells)
    Loading amount
    15 µg
    Specification
    many whole cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 10 2012

    Immunoprecipitation abreview for Anti-Ago1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Embryo)
    Total protein in input
    1 µg
    Specification
    Embryo
    Immuno-precipitation step
    Protein G
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 23 2010

    Western blot abreview for Anti-Ago1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Schmidtea mediterranea Tissue lysate - whole (many adult somatic stem cells)
    Loading amount
    21 µg
    Specification
    many adult somatic stem cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Dr. An Zeng

    Verified customer

    提交于 Mar 12 2010

    Question

    Have you ever tested Ago1 antibody in IP assay?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 22 2005

    Answer

    Thank you for your enquiry. No, to our knowledge this antiserum has not yet been applied using immunoprecipitation. I would be very interested to know how it performs and therefore I am prepared to provide you with a discount code should you wish to test it by IP. In return I would ask that you submit an Abreview by clicking on the "Abreviews" tab under the product datasheet. I look forward to hearing from you.

    Read More

    Abcam Scientific Support

    回复于 Nov 23 2005

    Question

    Customer purchased ab5070, lot# 64529, and it worked well in IP. Is that lot still available? If not, is the current lot from the same rabbit?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 02 2005

    Answer

    Thank you for your enquiry. Lot# 64529 is no longer available, but the current stock that we do have is from the same rabbit. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Aug 04 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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