Anti-Ago1抗体(ab5070)
Key features and details
- Rabbit polyclonal to Ago1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Drosophila melanogaster
- Isotype: IgG
概述
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产品名称
Anti-Ago1抗体
参阅全部 Ago1 一抗 -
描述
兔多克隆抗体to Ago1 -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Drosophila melanogaster -
免疫原
Synthetic peptide corresponding to Drosophila melanogaster Ago1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab23538) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (2) |
Use at an assay dependent concentration. PubMed: 18463631
|
| IHC-P |
Use at an assay dependent concentration. PubMed: 18528333
|
|
| WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa.
|
| 说明 |
|---|
|
IP
Use at an assay dependent concentration. PubMed: 18463631 |
|
IHC-P
Use at an assay dependent concentration. PubMed: 18528333 |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. |
靶标
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功能
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). -
序列相似性
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
细胞定位
Cytoplasm > P-body. - Information by UniProt
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数据库链接
- Entrez Gene: 36544 Drosophila melanogaster
- Entrez Gene: 236511 Mouse
- SwissProt: Q8CJG1 Mouse
- Unigene: 30800 Mouse
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别名
- AGO1 antibody
- AGO1_HUMAN antibody
- Argonaute 1 antibody
see all
图片
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Western blot - Anti-Ago1 antibody (ab5070)Anti-Ago1 antibody (ab5070) at 1 µg/ml + Drosophila Melanogaster embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Western blot - Anti-Ago1 antibody (ab5070)This image is courtesy of an anonymous AbreviewAnti-Ago1 antibody (ab5070) + Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg
Secondary
Sheep anti-rabbit HRP conjugated IgG Polyclonal
Performed under reducing conditions.
Predicted band size: 97 kDaBlot was blocked with 5% Milk for 1 hour at room temperature.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (44)
ab5070 被引用在 44 文献中.
- Rozen-Gagnon K et al. A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines. Sci Rep 11:736 (2021). PubMed: 33436886
- Kingston ER & Bartel DP Ago2 protects Drosophila siRNAs and microRNAs from target-directed degradation, even in the absence of 2'-O-methylation. RNA 27:710-724 (2021). PubMed: 33853897
- Iki T et al. Modulation of Ago2 Loading by Cyclophilin 40 Endows a Unique Repertoire of Functional miRNAs during Sperm Maturation in Drosophila. Cell Rep 33:108380 (2020). PubMed: 33176138
- Adiliaghdam F et al. A Requirement for Argonaute 4 in Mammalian Antiviral Defense. Cell Rep 30:1690-1701.e4 (2020). PubMed: 32049003
- Zhan J et al. lncRNA PVT1 aggravates doxorubicin-induced cardiomyocyte apoptosis by targeting the miR-187-3p/AGO1 axis. Mol Cell Probes 49:101490 (2020). PubMed: 31786333