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Agarose Anti-6X His tag®抗体(ab1231)

Submit a review Q&A (2)References (1)

Key features and details

  • Agarose Goat polyclonal to 6X His tag®
  • Suitable for: IP
  • Reacts with: Species independent
  • Conjugation: Agarose
  • Isotype: IgG

概述

  • 产品名称

    Agarose Anti-6X His tag®抗体
    参阅全部 6X His tag® 一抗

  • 描述

    Agarose山羊多克隆抗体to 6X His tag®

  • 宿主

    Goat

  • 偶联物

    Agarose

  • 经测试应用

    适用于: IPmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Full length native

    HHHHHH

    (6-His)(purified): conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1231于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent concentration.
Use 20-40 µl of gel slurry per 0.1-1 mg of protein lysate or extract.
说明
IP
Use at an assay dependent concentration.
Use 20-40 µl of gel slurry per 0.1-1 mg of protein lysate or extract.

靶标

  • 相关性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells.

  • 细胞定位

    Depends upon the localization of the parent protein tagged with hexahistidine.

  • 别名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

文献 (1)

发表研究结果有使用 ab1231？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab1231 被引用在 1 文献中.

  • Ma X  et al. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb. Elife 8:N/A (2019). PubMed: 30652970

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Ich habe bei Ihnen das Produkt ab 1231 erworben und hätte zu diesem
Produkt einige Fragen (Anti-6XHistag antibody, ..). Ich
möchte, wenn möglich, ein Proteinpartikel pro Bead anbringen. Hierfür
bräuchte ich die Konzentration der Beadpartikel/mL Lösung. Daher wäre
ich Ihnen sehr verbunden, wenn Sie mir diese mitteilen könnten.
Darüber hinaus würde ich es sehr zu schätzen wissen, wenn Sie mir
ungefähr die Größe eines Beadpartikels mitteilen könnten.

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Answer

Vielen Dank fürIhre Anfrage.

Das Labor hat bestätig,dass in 400ul Puffer (50% Slurry)es 200ul Beads hat(und 100ugAntikörper).

Hier ist ein Link zu den Beads, welchebenutzt wurden. Ich bin mir nicht sicher, wie Sie genau die Anzahl Partikel berechnen möchten/können. Bitte lassen Sie mich wissen, falls Sie mehr Informationen vom Labor bräuchten:...

Ich hoffe, dies hilft Ihnen trotzdemweiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Question

What type of agarose beads were used?

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Answer

The type of agarose beads that were used were - Uniflow 4 from Sterogene.

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