Key features and details
- Rat monoclonal [1C6] to Aflatoxin
- Suitable for: ELISA
- Reacts with: Species independent
- Isotype: IgG2b
描述大鼠单克隆抗体[1C6] to Aflatoxin
特异性This antibody reacts with Aflatoxin M1 (AFM1) and Aflatoxin B1 (AFB1). It does not react with BSA and other irrelevant antigens by ELISA. A competitive binding assay using AFM1 as the binding competitor to compete with AFM1-BSA-125I showed that AFM1 can effectively inhibit the binding of this antibody to AFM1-BSA-125I and the inhibition degree corresponds to the amounts of AFM1 used. The equilibrium constant K of antibody affinity of this product is 109-1011 M-1.
经测试应用适用于: ELISAmore details
种属反应性与反应: Species independent
Aflatoxin M1-BSA conjugate
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituent: 0.0268% PBS
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Our Abpromise guarantee covers the use of ab685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. This antibody is reactive to purified AFB1 and AF in samples obtained from cereals contaminated with fungus Aspergellus flarus. Therefore, this antibody can be used for construction of an AF detection kit.|
相关性Aflatoxins are naturally occurring mycotoxins that are produced by many species of Aspergillus.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab685 被引用在 4 文献中.
- Chalyan T et al. AFM1 Detection in Milk by Fab' Functionalized Si3N4 Asymmetric Mach-Zehnder Interferometric Biosensors. Toxins (Basel) 11:N/A (2019). PubMed: 31337103
- Kanungo L & Bhand S Fluorimetric immunoassay for multianalysis of aflatoxins. J Anal Methods Chem 2013:584964 (2013). ELISA . PubMed: 24000318
- Parker CO & Tothill IE Development of an electrochemical immunosensor for aflatoxin M1 in milk with focus on matrix interference. Biosens Bioelectron 24:2452-7 (2009). PubMed: 19167207
- Parker CO et al. Electrochemical immunochip sensor for aflatoxin M1 detection. Anal Chem 81:5291-8 (2009). PubMed: 19489595