Anti-Adiponectin抗体(ab3455)
Key features and details
- Rabbit polyclonal to Adiponectin
- Suitable for: ICC, WB
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Adiponectin抗体
参阅全部 Adiponectin 一抗 -
描述
兔多克隆抗体to Adiponectin -
宿主
Rabbit -
特异性
Detects Acrp30 from mouse serum. We have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Mouse -
免疫原
This product was produced with the following immunogens:
Synthetic peptide corresponding to Mouse Adiponectin aa 1-100.
Synthetic peptide corresponding to Mouse Adiponectin aa 150-250. -
阳性对照
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 2 µg/ml.
|
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| WB |
Use a concentration of 4 - 8 µg/ml. Detects a band of approximately 30 kDa.
|
| 说明 |
|---|
|
ICC
Use a concentration of 2 µg/ml. |
|
WB
Use a concentration of 4 - 8 µg/ml. Detects a band of approximately 30 kDa. |
靶标
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功能
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. -
组织特异性
Synthesized exclusively by adipocytes and secreted into plasma. -
疾病相关
Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. -
序列相似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
结构域
The C1q domain is commonly called the globular domain. -
翻译后修饰
Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 11450 Mouse
- SwissProt: Q60994 Mouse
- Unigene: 3969 Mouse
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别名
- 30 kDa adipocyte complement related protein antibody
- 30 kDa adipocyte complement-related protein antibody
- ACDC antibody
see all
图片
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Western blot - Anti-Adiponectin antibody (ab3455)All lanes : Anti-Adiponectin antibody (ab3455) at 2 µg/ml
Lane 1 : Mouse adipose tissue extract in 3T3-L1 conditioned media
Lane 2 : Mouse adipose tissue extract in NIH/3T3 conditioned media
Lane 3 : Mouse adipose tissue extract in BM-MSC differentiated conditioned media
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
Developed using the ECL technique. -
Immunocytochemistry - Anti-Adiponectin antibody (ab3455)Immunofluorescence analysis of Adiponectin was performed using 70% confluent 3T3-L1 cells differentiated with Adipogenesis Assay Kit (Cell-Based)(ab133102) for 5 days. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton TM X-100 for 10 min, blocked with 1% BSA for 1 hour at room temperature.
The cells were labeled with Adiponectin Polyclonal Antibody (ab3455) at 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then lebeled with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at a dilution of 1/2000 for 45 minutes at room temperature, panel A (green). Panel B (blue): nuclei were stained with DAPI. Panel C (red), F-actin was stained with Alexa Fluor®555 rhodamine phalloidin (ab235138) at 1/3000 dilution. Panel D represents the merged image showing cytoplasmic localization. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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Western blot - Anti-Adiponectin antibody (ab3455)Western blot detection of Adiponectin on mouse serum using ab3455.
数据表及文件
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Datasheet download
文献 (18)
ab3455 被引用在 18 文献中.
- Kim K et al. Adipocyte PHLPP2 inhibition prevents obesity-induced fatty liver. Nat Commun 12:1822 (2021). PubMed: 33758172
- Morris EV et al. Myeloma Cells Down-Regulate Adiponectin in Bone Marrow Adipocytes Via TNF-Alpha. J Bone Miner Res 35:942-955 (2020). PubMed: 31886918
- Li B et al. Metformin regulates adiponectin signalling in epicardial adipose tissue and reduces atrial fibrillation vulnerability. J Cell Mol Med 24:7751-7766 (2020). PubMed: 32441464
- Yuan X et al. The Effects of Brown Algae-Derived Monosaccharide L-Fucose on Lipid Metabolism in C57BL/6J Obese Mice. Nutrients 12:N/A (2020). PubMed: 33322300
- Zhu L et al. Hepatocyte estrogen receptor alpha mediates estrogen action to promote reverse cholesterol transport during Western-type diet feeding. Mol Metab 8:106-116 (2018). PubMed: 29331506
