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Cardiovascular Lipids / Lipoproteins Adipose Related Acrp

Anti-Adiponectin抗体(ab3455)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (14)

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Western blot - Anti-Adiponectin antibody (ab3455)

    Key features and details

    • Rabbit polyclonal to Adiponectin
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Adiponectin抗体
      参阅全部 Adiponectin 一抗
    • 描述

      兔多克隆抗体to Adiponectin
    • 宿主

      Rabbit
    • 特异性

      Detects Acrp30 from mouse serum. We have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data
    • 免疫原

      This product was produced with the following immunogens:
      Synthetic peptide corresponding to Mouse Adiponectin aa 1-100.

      Synthetic peptide corresponding to Mouse Adiponectin aa 150-250.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • 购买相配的WB阳性对照Recombinant Mouse Adiponectin (mutated C39A) protein
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cardiovascular
      • Lipids / Lipoproteins
      • Adipose Related
      • Acrp
      • Signal Transduction
      • Growth Factors/Hormones
      • Hormones
      • Neuroscience
      • Neurology process
      • Metabolism
      • Stem Cells
      • Mesenchymal Stem Cells
      • Adipogenesis
      • Cardiovascular
      • Atherosclerosis
      • Diabetes associated
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Diabetes
      • Metabolism
      • Types of disease
      • Obesity
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease
      • Metabolism
      • Types of disease
      • Metabolic disorders

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Adiponectin protein (ab51294)

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3455 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    应用 Species
    WB
    Mouse
    应用 Ab评论 说明
    WB
    Use a concentration of 4 - 8 µg/ml. Detects a band of approximately 30 kDa.
    说明
    WB
    Use a concentration of 4 - 8 µg/ml. Detects a band of approximately 30 kDa.

    靶标

    • 功能

      Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
    • 组织特异性

      Synthesized exclusively by adipocytes and secreted into plasma.
    • 疾病相关

      Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
      Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    • 序列相似性

      Contains 1 C1q domain.
      Contains 1 collagen-like domain.
    • 结构域

      The C1q domain is commonly called the globular domain.
    • 翻译后修饰

      Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
      HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
      O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
    • 细胞定位

      Secreted.
    • Target information above from: UniProt accession Q15848 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 11450 Mouse
      • SwissProt: Q60994 Mouse
      • Unigene: 3969 Mouse
      • 别名

        • 30 kDa adipocyte complement related protein antibody
        • 30 kDa adipocyte complement-related protein antibody
        • ACDC antibody
        • Acrp 30 antibody
        • ACRP30 antibody
        • ADIPO_HUMAN antibody
        • Adipocyte antibody
        • Adipocyte C1q and collagen domain containing protein antibody
        • Adipocyte complement related 30 kDa protein antibody
        • Adipocyte complement related protein of 30 kDa antibody
        • Adipocyte complement-related 30 kDa protein antibody
        • adipocyte-specific secretory protein antibody
        • Adiponectin antibody
        • Adiponectin precursor antibody
        • adiponectin, C1Q and collagen domain containing antibody
        • Adipoq antibody
        • Adipose most abundant gene transcript 1 antibody
        • Adipose most abundant gene transcript 1 protein antibody
        • Adipose specific collagen like factor antibody
        • ADIPQTL1 antibody
        • ADPN antibody
        • APM 1 antibody
        • apM-1 antibody
        • APM1 antibody
        • C1q and collagen domain-containing protein antibody
        • GBP 28 antibody
        • GBP28 antibody
        • Gelatin binding protein antibody
        • Gelatin binding protein 28 antibody
        • gelatin-binding protein 28 antibody
        • Gelatin-binding protein antibody
        • OTTHUMP00000210047 antibody
        see all

      图片

      • Western blot - Anti-Adiponectin antibody (ab3455)
        Western blot - Anti-Adiponectin antibody (ab3455)

        Western blot detection of Adiponectin on mouse serum using ab3455.

      实验方案

      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (14)

      发表研究结果有使用 ab3455?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3455 被引用在 14 文献中.

      • Zhu L  et al. Hepatocyte estrogen receptor alpha mediates estrogen action to promote reverse cholesterol transport during Western-type diet feeding. Mol Metab 8:106-116 (2018). PubMed: 29331506
      • Rödiger M  et al. Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes. Mol Metab 8:167-179 (2018). WB, ICC/IF ; Human . PubMed: 29203237
      • Bao L  et al. A long-acting FGF21 alleviates hepatic steatosis and inflammation in a mouse model of non-alcoholic steatohepatitis partly through an FGF21-adiponectin-IL17A pathway. Br J Pharmacol N/A:N/A (2018). PubMed: 29859019
      • Xia Y  et al. Adiponectin determines farnesoid X receptor agonism-mediated cardioprotection against post-infarction remodelling and dysfunction. Cardiovasc Res 114:1335-1349 (2018). PubMed: 29668847
      • Piao L  et al. Chronic Psychological Stress Accelerates Vascular Senescence and Impairs Ischemia-Induced Neovascularization: The Role of Dipeptidyl Peptidase-4/Glucagon-Like Peptide-1-Adiponectin Axis. J Am Heart Assoc 6:N/A (2017). PubMed: 28963101
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-3 of 3 Abreviews or Q&A

      Question

      I am interested in the adiponectin antibody. I was wondering if this antibody has been used for immunohistochemistry? I would love to try it out, but would like to know if you can work with us in such a way that if the assay does not work (we can send an image, our protocol, etc) could we get a refund or credit?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 03 2003

      Answer

      Our policy is that if an antibody does not work as specified on the datasheet, we will provide a replacement vial or a refund. However, this antibody has not yet been tested for use in IHC so we will not refund you if the antibody is not suitable for this application. Should you decide to go ahead and test this antibody in IHC, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher. Please note that this antibody does not cross react with human and only weakly with rat Acrp 30.

      Read More

      Abcam Scientific Support

      回复于 Oct 03 2003

      Question

      Dear Danielle Miller, Attached, please find a Powerpoint file with a scanned image of a Western blot using ab3455. Please send a replacement vial of ab6672 as soon as possible.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 17 2003

      Answer

      Thank you for sending the blot image. I shall now amend our datasheet to let other reaseachers interested in this antibody know that it does not cross react with human and only very poorly with rat. ab6672 -- Rabbit polyclonal to human IL-6 - will be sent to you on Monday of next week. There will be no charge for the product as agreed and we will also cover the shipping fees. The order number for this replacement item is 19951.

      Read More

      Abcam Scientific Support

      回复于 Jun 19 2003

      Question

      We have now tested your rabbit polyclonal antibody against Acrp 30 (ab3455) on Western blot. It gives a very strong and clean signal for mouse serum, no signal for human serum and only a very faint signal for rat serum. As we discussed when I ordered it, I wanted to use it for rat serum and you thought it would be OK. However, that is not the case Therefore, as we agreed upon, I would like to have a replacement antibody, namely a rabbit polyclonal against IL-6 (ab6672). Please ship that as soon as possible. If it can be off any use to you, I will be happy to send you an image of our Western blot.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 13 2003

      Answer

      I recall our telephone conversation in April where I agreed that should you find that ab3455 Rabbit polyclonal to Acrp 30 does not cross react with rat then we would send an alternative product. I am very surprised that this antibody does not work well with rat and will of course send you a replacement vial of ab6672 as requested. I would like to see your WB image for mouse, rat and human serum. Did you load the sample amount of protein in all three cases? ab6672 is in stock and I shall arrange for the order to be despatched to you as soon as I have the blot image for ab3455.

      Read More

      Abcam Scientific Support

      回复于 Jun 16 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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