Anti-Adiponectin抗体(ab216502)
Key features and details
- Rabbit polyclonal to Adiponectin
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Anti-Adiponectin抗体
参阅全部 Adiponectin 一抗 -
描述
兔多克隆抗体to Adiponectin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Adiponectin aa 140-190 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q15848 -
阳性对照
- IHC-P: Human colon carcinoma and mouse liver tissues. WB: Rat liver, mouse intestine, heart and spleen lysates. HepG2 cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
研究领域
相关产品
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Compatible Secondaries
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab216502于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/500.
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WB |
1/100 - 1/1000. Predicted molecular weight: 26 kDa.
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说明 |
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IHC-P
1/100 - 1/500. |
WB
1/100 - 1/1000. Predicted molecular weight: 26 kDa. |
靶标
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功能
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. -
组织特异性
Synthesized exclusively by adipocytes and secreted into plasma. -
疾病相关
Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. -
序列相似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
结构域
The C1q domain is commonly called the globular domain. -
翻译后修饰
Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 9370 Human
- Entrez Gene: 11450 Mouse
- Entrez Gene: 246253 Rat
- Omim: 605441 Human
- SwissProt: Q15848 Human
- SwissProt: Q60994 Mouse
- Unigene: 80485 Human
- Unigene: 3969 Mouse
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别名
- 30 kDa adipocyte complement related protein antibody
- 30 kDa adipocyte complement-related protein antibody
- ACDC antibody
see all
图片
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Anti-Adiponectin antibody (ab216502) at 1/300 dilution + HepG2 cell lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 26 kDa -
Anti-Adiponectin antibody (ab216502) at 1/300 dilution + Mouse spleen lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 26 kDa -
Anti-Adiponectin antibody (ab216502) at 1/300 dilution + mouse heart lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 26 kDa -
All lanes : Anti-Adiponectin antibody (ab216502) at 1/200 dilution
Lane 1 : rat liver lysate
Lane 2 : mouse intestine lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 26 kDa -
Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse liver tissue labeling Adiponectin using ab216502 at 1/200, followed by secondary detection and DAB staining.
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Immunohistochemical analysis of formalin-fixed and paraffin embedded human colon carcinoma tissue labeling Adiponectin using ab216502 at 1/200, followed by secondary detection and DAB staining.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab216502 尚未被引用在任何文献中。