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Anti-Adenosine Receptor A2a抗体(ab3461)

  • Datasheet
Reviews (2)Q&A (1)References (19)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)

Key features and details

  • Rabbit polyclonal to Adenosine Receptor A2a
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Dog, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Adenosine Receptor A2a抗体
    参阅全部 Adenosine Receptor A2a 一抗
  • 描述

    兔多克隆抗体to Adenosine Receptor A2a
  • 宿主

    Rabbit
  • 特异性

    Detects adenosine receptor A2a. This antibody does not detect other AR subtypes.
  • 经测试应用

    适用于: ICC, WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Dog, Human
    预测可用于: Horse
  • 免疫原

    Synthetic peptide corresponding to Dog Adenosine Receptor A2a aa 373-391.
    Sequence:

    ESHGDMGLPDVELLSHELK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Cardiovascular
    • Blood
    • Coagulation
    • Regulatory
    • Cell Biology
    • Apoptosis
    • Phagocytosis
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Phagocytosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Adenosine Receptor A2a protein (ab126024)

应用

Our Abpromise guarantee covers the use of ab3461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
WB 1/1000.
IHC-P 1/20 - 1/200.

靶标

  • 功能

    Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • 序列相似性

    Belongs to the G-protein coupled receptor 1 family.
  • 结构域

    The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.
  • 翻译后修饰

    Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession P29274 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 135 Human
    • Entrez Gene: 11540 Mouse
    • Entrez Gene: 25369 Rat
    • Omim: 102776 Human
    • SwissProt: P11617 Dog
    • SwissProt: Q6TLI7 Horse
    • SwissProt: P29274 Human
    • SwissProt: Q60613 Mouse
    • SwissProt: P30543 Rat
    • Unigene: 197029 Human
    • Unigene: 333734 Mouse
    • Unigene: 11180 Rat
    see all
  • 别名

    • A2AAR antibody
    • A2aR antibody
    • AA2AR_HUMAN antibody
    • ADENO antibody
    • Adenosine A2 receptor antibody
    • Adenosine A2a receptor antibody
    • Adenosine receptor A2a antibody
    • Adenosine receptor subtype A2a antibody
    • ADORA 2 antibody
    • ADORA2 antibody
    • ADORA2A antibody
    • hA2aR antibody
    • RDC 8 antibody
    • RDC8 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)

    ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Mouse testis tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)
    Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)
    All lanes : Anti-Adenosine Receptor A2a antibody (ab3461) at 1/500 dilution

    Lane 1 : Human placenta cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Mouse liver cell lysate

    Lysates/proteins at 25 µg per lane.
  • Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)
    Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)

    Immunocytochemistry/Immunofluorescence analysis of Adenosine Receptor A2a (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3461 in 3% BSA-PBS at a dilution of 1:20 overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)

    ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Human testis tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)

    ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Human placenta tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)
    Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)This image is courtesy of an anonymous Abreview
    Anti-Adenosine Receptor A2a antibody (ab3461) at 1/1000 dilution + Human heart tissue lysate at 20 µg

    Secondary
    Donkey HRP-conjugate anti-rabbit at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 3 minutes

    See Abreview

  • Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)
    Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)
    ICC/IF image of ab3461 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3461, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (19)

    发表研究结果有使用 ab3461?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3461 被引用在 19 文献中.

    • Castro CM  et al. Adenosine A2A receptor (A2AR) stimulation enhances mitochondrial metabolism and mitigates reactive oxygen species-mediated mitochondrial injury. FASEB J 34:5027-5045 (2020). PubMed: 32052890
    • Schröder S  et al. PET Imaging of the Adenosine A2A Receptor in the Rotenone-Based Mouse Model of Parkinson's Disease with [18F]FESCH Synthesized by a Simplified Two-Step One-Pot Radiolabeling Strategy. Molecules 25:N/A (2020). PubMed: 32252340
    • Xu K  et al. Adenosine from a biologic source regulates neutrophil extracellular traps (NETs). J Leukoc Biol 105:1225-1234 (2019). PubMed: 30907983
    • Han YY  et al. Cordycepin improves behavioral-LTP and dendritic structure in hippocampal CA1 area of rats. J Neurochem 151:79-90 (2019). PubMed: 31314908
    • Gordian E  et al. Transforming growth factor ß-induced epithelial-to-mesenchymal signature predicts metastasis-free survival in non-small cell lung cancer. Oncotarget 10:810-824 (2019). PubMed: 30783512
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    ELISA abreview for Anti-Adenosine Receptor A2a antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Cell (Cell Membrane)
    Specification
    Cell Membrane
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Type
    Sandwich (Detection)
    Read More

    Tammy Zhai

    Verified customer

    提交于 Apr 22 2015

    Western blot

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8)
    Sample
    Human Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 10 2013

    Question

      A researcher from Mexico needs to use a Anti-Adenosine Receptor A2a antibody in rat and CoIP. I found ab3461 but it has not been tested for rat. Could you check the homology to see if you would recommend trying this antibody and if the 100% testing discount would apply. He also needs Anti-Dopamine D2 Receptor antibody to use in rat and CoIP. I found ab21218 that has been tested in rat and IP, but he wants to know if it will work in CoIP as well.  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 26 2011

    Answer

    Hi Daniela, I did an alignment between the rat sequence and the immnunogen sequence for ab3461 and I would not recommend using this antibody in rat. The level of similarity between the two is to low. I would however recommend ab101678, it has not been tested in IP, but it will react with rat tissue. The customer could get a testing discount for trying it out in IP. As for ab21218, I think that one should work in CoIP, if it works in IP, it should work for CoIP. Let me know if there is anything else I can help with.  

    Read More

    Abcam Scientific Support

    回复于 Oct 26 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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