Anti-Adenosine Receptor A2a抗体[7F6-G5-A2] (ab79714)
Key features and details
- Mouse monoclonal [7F6-G5-A2] to Adenosine Receptor A2a
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
概述
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产品名称
Anti-Adenosine Receptor A2a抗体[7F6-G5-A2]
参阅全部 Adenosine Receptor A2a 一抗 -
描述
小鼠单克隆抗体[7F6-G5-A2] to Adenosine Receptor A2a -
宿主
Mouse -
特异性
This antibody is recommended for tissue lysates only. In house testing has shown no signal in Western Blot for SH-SY5Y, SK-N-SH, PC-12 or HeLa cell lines.
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经测试应用
适用于: IHC-P, WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Guinea pig, Hamster, Dog, Non human primates -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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表位
Recognises amino acids 213-220 (SQPLPGER) within the third intracellular loop. -
阳性对照
- WB: Human, mouse, and rat brain tissue lysates. IHC-P: Rat, mouse and human brain caudate nucleus.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified by running the antiserum from the injected animal through an affinity column with the antigen bound to a beaded agarose gel. -
克隆
单克隆 -
克隆编号
7F6-G5-A2 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79714于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).
We recommend using 1% BSA as a blocking agent for western blot. |
说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa). We recommend using 1% BSA as a blocking agent for western blot. |
靶标
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功能
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
结构域
The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface. -
翻译后修饰
Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 135 Human
- Entrez Gene: 11540 Mouse
- Entrez Gene: 25369 Rat
- Omim: 102776 Human
- SwissProt: P11617 Dog
- SwissProt: P29274 Human
- SwissProt: Q60613 Mouse
- SwissProt: P30543 Rat
see all -
别名
- A2AAR antibody
- A2aR antibody
- AA2AR_HUMAN antibody
see all
图片
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IHC image of Adenosine Receptor A2a staining in Mouse normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Lanes 1-3 : Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714) at 5 µg/ml
Lanes 4-6 : Competitor product at 5 µg/ml
Lanes 1 & 4 : Human brain tissue lysate
Lanes 2 & 5 : Mouse brain tissue lysate
Lanes 3 & 6 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Additional bands at: 25 kDa, 39 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesBlocking buffer: 1% BSA
Gel type: MOPS
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IHC image of Adenosine Receptor A2a staining in Human normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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IHC image of Adenosine Receptor A2a staining in Rat normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab79714 被引用在 6 文献中.
- Stocco E et al. Experimental Evidence of A2A-D2 Receptor-Receptor Interactions in the Rat and Human Carotid Body. Front Physiol 12:645723 (2021). PubMed: 33935801
- Sánchez-Melgar A et al. Adenosine and Metabotropic Glutamate Receptors Are Present in Blood Serum and Exosomes from SAMP8 Mice: Modulation by Aging and Resveratrol. Cells 9:N/A (2020). PubMed: 32645849
- Huang W et al. An adenosine A1R-A2aR imbalance regulates low glucose/hypoxia-induced microglial activation, thereby contributing to oligodendrocyte damage through NF-?B and CREB phosphorylation. Int J Mol Med 41:3559-3569 (2018). PubMed: 29512780
- Sakano D et al. Dopamine D2 Receptor-Mediated Regulation of Pancreatic ß Cell Mass. Stem Cell Reports 7:95-109 (2016). Mouse . PubMed: 27373926
- Niesman IR et al. Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin deficient mice. J Neuroinflammation 11:39 (2014). WB ; Mouse . PubMed: 24593993
- Bone DB et al. Nucleoside/nucleobase transport and metabolism by microvascular endothelial cells isolated from ENT1-/- mice. Am J Physiol Heart Circ Physiol 299:H847-56 (2010). WB ; Mouse . PubMed: 20543083