Key features and details
- Rabbit polyclonal to Adenosine A1 Receptor
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
纯度Immunogen affinity purified
Primary antibody说明Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab3460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
功能Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
序列相似性Belongs to the G-protein coupled receptor 1 family.
- Information by UniProt
- A1AR antibody
- A1R antibody
- AA1R antibody
ab3460 labelling Adenosine A1 receptor in the cytoplasm of Rat brain tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
ab3460 被引用在 3 文献中.
- Stettler O et al. Engrailed homeoprotein recruits the adenosine A1 receptor to potentiate ephrin A5 function in retinal growth cones. Development 139:215-24 (2012). PubMed: 22147955
- Datino T et al. Mechanisms by which adenosine restores conduction in dormant canine pulmonary veins. Circulation 121:963-72 (2010). WB ; Dog . PubMed: 20159830
- Kempson SA et al. Acute inhibition of the betaine transporter by ATP and adenosine in renal MDCK cells. Am J Physiol Renal Physiol 295:F108-17 (2008). WB ; Dog . PubMed: 18448594