Anti-ADAMTS13抗体(ab28273)
Key features and details
- Rabbit polyclonal to ADAMTS13
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ADAMTS13抗体
参阅全部 ADAMTS13 一抗 -
描述
兔多克隆抗体to ADAMTS13 -
宿主
Rabbit -
特异性
ab28273 recognises the Carboxyterminal end of the long form of ADAMTS13. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide based on the carboxyterminal end of full length human ADAMTS13. (Peptide available as ab41251.)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28273于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
1/1000 - 1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa). 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Detects a band of approximately 190 kDa. Glycosylation and the abundance of cysteine residues gives ADAMTS13 an apparent molecular weight of 190 kDa on reduced SDS PAGE gels. Several bands between 110-190 kDa are observed on Western blots, possibly indicating differentially processed ADAMTS 13. Dilution optimised using Chromogenic detection.
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说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
1/1000 - 1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa). 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Detects a band of approximately 190 kDa. Glycosylation and the abundance of cysteine residues gives ADAMTS13 an apparent molecular weight of 190 kDa on reduced SDS PAGE gels. Several bands between 110-190 kDa are observed on Western blots, possibly indicating differentially processed ADAMTS 13. Dilution optimised using Chromogenic detection. |
靶标
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功能
Cleaves the vWF multimers in plasma into smaller forms. -
组织特异性
Plasma. Expressed primarily in liver. -
疾病相关
Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. -
序列相似性
Contains 2 CUB domains.
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 8 TSP type-1 domains. -
结构域
The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction. -
翻译后修饰
May contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
The precursor is processed by a furin endopeptidase which cleaves off the pro-domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 11093 Human
- Omim: 604134 Human
- SwissProt: Q76LX8 Human
- Unigene: 131433 Human
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别名
- A disintegrin and metalloproteinase with thrombospondin motifs 13 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 antibody
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 13 antibody
see all
图片
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All lanes : Anti-ADAMTS13 antibody (ab28273) at 1 µg/ml
Lane 1 : Recombinant Human ATS-13 at 0.08 µg
Lane 2 : Recombinant Human ATS-13 at 0.04 µg
Lane 3 : Recombinant Human ATS-13 at 0.02 µg
Predicted band size: 154 kDa
Glycosylation and the abundance of cysteine residues gives ADAMTS-13 an apparent molecular weight of about 190 kDa on reduced SDS PAGE gels. -
ICC/IF image of ab28273 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28273, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
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Datasheet download
文献 (0)
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