Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (kidney)
Total protein in input
500 µg
Immuno-precipitation step
Protein A
Specification
kidney
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: RIPA lysis buffer
Western Blot antibody
Name
Abcam antibody:Anti-ADAM10 antibody
Additional data
Additional Notes
Samples used: Kidney tissues
Lysis buffer used: RIPA + protease inhibitors
500ug protein for IP incubated with primary antibody Anti-ADAM10 antibody (ab1997)
80ug protein loaded in SDS-PAGE gel (10% poly acrylamide gel)
First antibody used in western blot: Anti-ADAM10 antibody (ab1997)
1, 2, 3, 4 lanes: kidney samples from four different individual mice
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Nora Alghamdi
Verified customer
提交于 Nov 22 2018