Anti-ADAM10抗体(ab1997)
Key features and details
- Rabbit polyclonal to ADAM10
- Suitable for: IHC-P, WB, ICC, ELISA, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-ADAM10抗体
参阅全部 ADAM10 一抗 -
描述
兔多克隆抗体to ADAM10 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC, ELISA, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Pig
-
免疫原
Synthetic peptide corresponding to Human ADAM10 aa 732-748.
Sequence:PQRQRPRESYQMGHMRR
(Peptide available asab7868) -
阳性对照
- WB: Jurkat whole cell lysate (ab7899). ICC: K562 cells. ICC/IF: HeLa and K562 cells.
-
常规说明
TNFa converting enzyme.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab1997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/500. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 84 kDa.Can be blocked with ADAM10 peptide (ab7868). | |
| ICC | Use a concentration of 2 µg/ml. | |
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 2 µg/ml. |
靶标
-
功能
Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. -
组织特异性
Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver. -
序列相似性
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
The precursor is cleaved by a furin endopeptidase. -
细胞定位
Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi. - Information by UniProt
-
数据库链接
- Entrez Gene: 282132 Cow
- Entrez Gene: 102 Human
- Entrez Gene: 11487 Mouse
- Entrez Gene: 397345 Pig
- Entrez Gene: 29650 Rat
- Omim: 602192 Human
- SwissProt: Q10741 Cow
- SwissProt: O14672 Human
see all -
别名
- A disintegrin and metalloprotease domain 10 antibody
- A disintegrin and metalloproteinase domain 10 antibody
- AD 10 antibody
see all
图片
-
Western blot - Anti-ADAM10 antibody (ab1997)Lane 1 : Anti-ADAM10 antibody (ab1997) at 1 µg/ml
Lane 2 : Anti-ADAM10 antibody (ab1997) at 2 µg/ml
All lanes : Jurkat whole cell lysate
Predicted band size: 84 kDa -
Immunocytochemistry - Anti-ADAM10 antibody (ab1997)ab1997 at 2µg/ml staining K562 cells by ICC/IF. -
Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody (ab1997)ICC/IF image of ab1997 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1997, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-ADAM10 antibody (ab1997)This image is courtesy of an anonymous AbreviewAll lanes : Anti-ADAM10 antibody (ab1997) at 1/1666 dilution
Lane 2 : ADAM10 siRNA treated human keratinocyte cells
Lane 3 : Non-treated human keratinocyte cells.
Secondary
All lanes : HRP conjugated Goat anti-rabbit antibody
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 100,75 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
-
Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody (ab1997)Immunofluorescence of ADAM10 in K562 cells using ab1997 at 10 ug/ml. -
Western blot - Anti-ADAM10 antibody (ab1997)This image is courtesy of an Abreview submitted by Grant CorbettAll lanes : Anti-ADAM10 antibody (ab1997) at 1/1000 dilution
All lanes : Human frontal cortex tissue lysate - whole
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye® 800CW-conjugated donkey anti-rabbit IgG polyclonal at 1/18000 dilution
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 65,80 kDa why is the actual band size different from the predicted?
Exposure time: 330 seconds
文献
ab1997 被引用在 74 文献中.
- Tang X et al. LncRNA ZFAS1 confers inflammatory responses and reduces cholesterol efflux in atherosclerosis through regulating miR-654-3p-ADAM10/RAB22A axis. Int J Cardiol N/A:N/A (2020). PubMed: 32349937
- Tang SY et al. Caspr1 Facilitates sAPPa Production by Regulating a-Secretase ADAM9 in Brain Endothelial Cells. Front Mol Neurosci 13:23 (2020). PubMed: 32210761
- Colas J et al. Neuroprotection against Amyloid-ß-Induced DNA Double-Strand Breaks Is Mediated by Multiple Retinoic Acid-Dependent Pathways. Neural Plast 2020:9369815 (2020). PubMed: 32256561
- Xiang XJ et al. Mitochondrial methionine sulfoxide reductase B2 links oxidative stress to Alzheimer's disease-like pathology. Exp Neurol 318:145-156 (2019). PubMed: 31078523
- Gao J et al. Long noncoding LINC01551 promotes hepatocellular carcinoma cell proliferation, migration, and invasion by acting as a competing endogenous RNA of microRNA-122-5p to regulate ADAM10 expression. J Cell Biochem 120:16393-16407 (2019). PubMed: 31270840
