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Cell Biology Apoptosis Receptors Receptor Processing

Anti-ADAM10抗体(ab1997)

  • Datasheet
  • SDS
Reviews (22)Q&A (4)References (79)

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Western blot - Anti-ADAM10 antibody (ab1997)
  • Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
  • Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
  • Western blot - Anti-ADAM10 antibody (ab1997)
  • Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
  • Western blot - Anti-ADAM10 antibody (ab1997)

Key features and details

  • Rabbit polyclonal to ADAM10
  • Suitable for: WB, ICC
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-ADAM10抗体
    参阅全部 ADAM10 一抗
  • 描述

    兔多克隆抗体to ADAM10
  • 宿主

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Human ADAM10 aa 732-748.
    Sequence:

    PQRQRPRESYQMGHMRR


    (Peptide available as ab7868)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    TNFa converting enzyme.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Receptors
    • Receptor Processing
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • ADAM Protein Family
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • ADAM protein family
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • ADAMs
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Receptor Processing

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • ADAM10 peptide (ab7868)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human ADAM10 protein (ab82822)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC
Human
WB
Human
All applications
Rat
Cow
Pig
应用 Ab评论 说明
WB (8)
1/500 - 1/2000. Predicted molecular weight: 84 kDa.Can be blocked with ADAM10 peptide (ab7868).
ICC (1)
Use a concentration of 2 µg/ml.
说明
WB
1/500 - 1/2000. Predicted molecular weight: 84 kDa.Can be blocked with ADAM10 peptide (ab7868).
ICC
Use a concentration of 2 µg/ml.

靶标

  • 功能

    Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
    -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
  • 组织特异性

    Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
  • 序列相似性

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • 结构域

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰

    The precursor is cleaved by a furin endopeptidase.
  • 细胞定位

    Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
  • Target information above from: UniProt accession O14672 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 282132 Cow
    • Entrez Gene: 102 Human
    • Entrez Gene: 397345 Pig
    • Entrez Gene: 29650 Rat
    • Omim: 602192 Human
    • SwissProt: Q10741 Cow
    • SwissProt: O14672 Human
    • SwissProt: O77633 Pig
    • SwissProt: Q10743 Rat
    • Unigene: 578508 Human
    • Unigene: 42924 Rat
    see all
  • 别名

    • A disintegrin and metalloprotease domain 10 antibody
    • A disintegrin and metalloproteinase domain 10 antibody
    • AD 10 antibody
    • AD10 antibody
    • AD18 antibody
    • ADA10_HUMAN antibody
    • ADAM 10 antibody
    • ADAM metallopeptidase domain 10 antibody
    • ADAM10 antibody
    • CD 156c antibody
    • CD156c antibody
    • CD156c antigen antibody
    • CDw156 antibody
    • disintegrin and metalloproteinase domain containing protein 10 antibody
    • Disintegrin and metalloproteinase domain-containing protein 10 antibody
    • HsT 18717 antibody
    • HsT18717 antibody
    • Kuz antibody
    • Kuzbanian antibody
    • Kuzbanian protein homolog antibody
    • Kuzbanian, Drosophila, homolog of antibody
    • MADM antibody
    • Mammalian disintegrin metalloprotease antibody
    • Mammalian disintegrin-metalloprotease antibody
    • RAK antibody
    see all

图片

  • Western blot - Anti-ADAM10 antibody (ab1997)
    Western blot - Anti-ADAM10 antibody (ab1997)
    Lane 1 : Anti-ADAM10 antibody (ab1997) at 1 µg/ml
    Lane 2 : Anti-ADAM10 antibody (ab1997) at 2 µg/ml

    All lanes : Jurkat whole cell lysate

    Predicted band size: 84 kDa

  • Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
    Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
    ab1997 at 2µg/ml staining K562 cells by ICC/IF.
  • Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
    Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
    ICC/IF image of ab1997 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1997, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-ADAM10 antibody (ab1997)
    Western blot - Anti-ADAM10 antibody (ab1997)This image is courtesy of an anonymous Abreview
    All lanes : Anti-ADAM10 antibody (ab1997) at 1/1666 dilution

    Lane 2 : ADAM10 siRNA treated human keratinocyte cells
    Lane 3 : Non-treated human keratinocyte cells.

    Secondary
    All lanes : HRP conjugated Goat anti-rabbit antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 84 kDa
    Observed band size: 100,75 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
    Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
    Immunofluorescence of ADAM10 in K562 cells using ab1997 at 10 ug/ml.

  • Western blot - Anti-ADAM10 antibody (ab1997)
    Western blot - Anti-ADAM10 antibody (ab1997)This image is courtesy of an Abreview submitted by Grant Corbett
    All lanes : Anti-ADAM10 antibody (ab1997) at 1/1000 dilution

    All lanes : Human frontal cortex tissue lysate - whole

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : IRDye® 800CW-conjugated donkey anti-rabbit IgG polyclonal at 1/18000 dilution

    Performed under reducing conditions.

    Predicted band size: 84 kDa
    Observed band size: 65,80 kDa why is the actual band size different from the predicted?


    Exposure time: 330 seconds

    See Abreview

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (79)

发表研究结果有使用 ab1997?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab1997 被引用在 79 文献中.

  • Tang X  et al. LncRNA ZFAS1 confers inflammatory responses and reduces cholesterol efflux in atherosclerosis through regulating miR-654-3p-ADAM10/RAB22A axis. Int J Cardiol N/A:N/A (2020). PubMed: 32349937
  • Tang SY  et al. Caspr1 Facilitates sAPPa Production by Regulating a-Secretase ADAM9 in Brain Endothelial Cells. Front Mol Neurosci 13:23 (2020). PubMed: 32210761
  • Colas J  et al. Neuroprotection against Amyloid-ß-Induced DNA Double-Strand Breaks Is Mediated by Multiple Retinoic Acid-Dependent Pathways. Neural Plast 2020:9369815 (2020). PubMed: 32256561
  • Hu T  et al. A novel rhamnoside derivative PL402 up-regulates matrix metalloproteinase 3/9 to promote Aß degradation and alleviates Alzheimer's-like pathology. Aging (Albany NY) 12:481-501 (2020). PubMed: 31901901
  • Seki T  et al. Galectin 3-binding protein suppresses amyloid-ß production by modulating ß-cleavage of amyloid precursor protein. J Biol Chem 295:3678-3691 (2020). PubMed: 31996371
View all Publications for this product

客户评价及客户问答

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1-10 of 26 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ADAM10 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Choroid plexus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Specification
Choroid plexus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Jan 22 2021

Immunohistochemistry (Frozen sections) abreview for Anti-ADAM10 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Choroid plexus)
Permeabilization
Yes - EDTA
Specification
Choroid plexus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Jan 22 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-ADAM10 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Glia cells)
Permeabilization
No
Specification
Glia cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Acetone
Read More

Mrs. Simone Grad

Verified customer

提交于 Oct 28 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-ADAM10 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Melanoma cells)
Permeabilization
No
Specification
Melanoma cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

提交于 Jul 10 2020

Immunocytochemistry abreview for Anti-ADAM10 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Liver cells)
Permeabilization
No
Specification
Liver cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

提交于 Jul 06 2020

Immunohistochemistry (Frozen sections) abreview for Anti-ADAM10 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Melanoma)
Permeabilization
No
Specification
Melanoma
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

提交于 Jun 26 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ADAM10 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH = 8
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Jun 26 2020

Immunoprecipitation abreview for Anti-ADAM10 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (kidney)
Total protein in input
500 µg
Immuno-precipitation step
Protein A
Specification
kidney
Read More

Dr. Nora Alghamdi

Verified customer

提交于 Nov 22 2018

Western blot abreview for Anti-ADAM10 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT-116, RERF-LC-Ad1)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HCT-116, RERF-LC-Ad1
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Nov 10 2017

Western blot abreview for Anti-ADAM10 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (4L melanoma cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
4L melanoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Praveen Agrawal

Verified customer

提交于 Mar 24 2017

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