重组
RabMAb

Anti-ACTN3抗体[EP2531Y] (ab68204)

概述

  • 产品名称
    Anti-ACTN3抗体[EP2531Y]
    参阅全部 ACTN3 一抗
  • 描述
    兔单克隆抗体[EP2531Y] to ACTN3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WB, IHC-P, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human ACTN3 (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • Human skeletal muscle lysate and human muscle tissue
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab68204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/5000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
    • 组织特异性
      Expressed only in a subset of type 2 skeletal muscle fibers.
    • 序列相似性
      Belongs to the alpha-actinin family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
      Contains 4 spectrin repeats.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Actinin alpha 3 antibody
      • actinin, alpha antibody
      • ACTN 3 antibody
      • ACTN3 antibody
      • ACTN3_HUMAN antibody
      • Alpha actinin skeletal muscle antibody
      • Alpha actinin skeletal muscle isoform 3 antibody
      • Alpha-Actinin 3 antibody
      • Alpha-actinin skeletal muscle isoform 3 antibody
      • Alpha-actinin-3 antibody
      • F actin cross linking protein antibody
      • F-actin cross-linking protein antibody
      • MGC117002 antibody
      • MGC117005 antibody
      • OTTHUMP00000219049 antibody
      • OTTHUMP00000219054 antibody
      see all

    图片

    • Anti-ACTN3 antibody [EP2531Y] (ab68204) at 1/10000 dilution + human skeletal muscle lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 103 kDa
      Observed band size: 103 kDa

    • ab68204 staining ACTN3 in human pluripotent stem cell derived cardiomyocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded human muscle tissue using ab68204 at 1/100-1/250 dilution.

    文献

    ab68204 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Thank you for contacting us.
    Skeletal Muscle (Human) Tissue Lysate is recommended as a positive control for ab68204. Both products are recommended for Western Blotting, and are guaranteed to work in this application.
    If you had any problems u...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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