Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
Anti-ACTN3 antibody [EP2531Y] (ab68204) at 1/10000 dilution + human skeletal muscle lysate at 10 µg
Secondary HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 103 kDa Observed band size: 103 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ACTN3 antibody [EP2531Y] (ab68204)This image is courtesy of an anonymous Abreview
ab68204 staining ACTN3 in human pluripotent stem cell derived cardiomyocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.