重组人ERK1蛋白(ab116536)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% Proprietary Purification
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, MS
描述
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产品名称
重组人ERK1蛋白
参阅全部 ERK1 蛋白酶 -
生物活性
Specific activity: 379.25 units/ml; 621.73 Units/mg
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纯度
> 80 % Proprietary Purification. -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAAAAAQGGGGGEPRRTEGV GPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAI KKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYI VQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS NLLSNTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSK GYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLN CIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRIT VEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETAR FQPGVLEAP -
预测分子量
70 kDa including tags -
氨基酸
2 to 379
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab116536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
Mass Spectrometry
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质谱法
MALDI-TOF-TOF -
形式
Liquid -
补充说明
ab116536 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF. The peptides obtained gave 27% sequence coverage of ERK1. Theoretical pI: 6.06 -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.002% Brij, 0.012% Benzamidine, 0.003% EGTA, 0.003% PMSF, 0.1% Beta mercaptoethanol, 0.79% Tris HCl, 9.24% Sucrose, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- ERK 1
- ERK-1
- ERK1
see all -
功能
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab116536 被引用在 2 文献中.
- Sinkala E & Herr AE Single-Cell Western Blotting. Methods Mol Biol 1346:1-9 (2015). PubMed: 26542711
- Hughes AJ et al. Single-cell western blotting. Nat Methods 11:749-55 (2014). PubMed: 24880876