重组Anti-Cleaved Caspase-3抗体[EPR21032] - BSA and Azide free (ab231289)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21032] to Cleaved Caspase-3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-Cleaved Caspase-3抗体[EPR21032] - BSA and Azide free
参阅全部 Cleaved Caspase-3 一抗 -
描述
兔单克隆抗体[EPR21032] to Cleaved Caspase-3 - BSA and Azide free -
宿主
Rabbit -
特异性
ab214430 recognises both pro-Caspase-3 and p17 cleavage fragments. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: NIH/3T3 cells treated with staurosporine, whole cell lysate.
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常规说明
ab231289 is the carrier-free version of ab214430.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR21032 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab231289于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 32, 29, 24, 19, 17 kDa (predicted molecular weight: 31 kDa).
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 32, 29, 24, 19, 17 kDa (predicted molecular weight: 31 kDa). |
靶标
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功能
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. -
组织特异性
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. -
序列相似性
Belongs to the peptidase C14A family. -
翻译后修饰
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 12367 Mouse
- SwissProt: P70677 Mouse
- Unigene: 34405 Mouse
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别名
- active caspase 3 antibody
- Apopain antibody
- CASP 3 antibody
see all
图片
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All lanes : Anti-Cleaved Caspase-3 antibody [EPR21032] (ab214430) at 1/5000 dilution
Lane 1 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 cells treated with 1 uM staurosprine for 4 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 14,19,24,29,32 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
Observed molecular masses are consistent with the literature for full-length and cleaved caspase 3 (PMID: 9922454, PMID 16221205).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214430).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (1)
ab231289 被引用在 1 文献中.
- Shen B et al. LncRNA GAS5 Silencing Attenuates Oxygen-Glucose Deprivation/Reperfusion-Induced Injury in Brain Microvascular Endothelial Cells via miR-34b-3p-Dependent Regulation of EPHA4. Neuropsychiatr Dis Treat 17:1667-1678 (2021). PubMed: 34079264