Anti-Caspase-3抗体(ab49822)
Key features and details
- Rabbit polyclonal to Caspase-3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Caspase-3抗体
参阅全部 Caspase-3 一抗 -
描述
兔多克隆抗体to Caspase-3 -
宿主
Rabbit -
特异性
The immunogen for this product is aa167-175 within the p17 subunit (cleaved caspase) but because this is a poly the epitope will vary and not all batches will be flush against the cleavage site.
This means it will detect a number of additional cleavage products in addition to p17:
• P12+17,
• P12+P17+propeptide 1
• p12+p17+proppetide 1 + propeptide 2,
• P17+propeptide 1,
• p17+propeptide 1 + propeptide 2 -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Caspase-3 aa 150-250 conjugated to keyhole limpet haemocyanin.
Database link: P42574 -
阳性对照
- ICC/IF: Human epitheloid carcinoma HeLa cell line, treated with staurosporine and WB: Human recombinant caspase-3, active.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
-
纯度
IgG fraction -
纯化说明
Whole antiserum is fractionated and then further purified by ion exchange chromatography. The resulting IgG fraction is further purified by absorption on the Caspase-3 peptide (Human, amino acids 167-178) corresponding to the uncleaved Caspase 3 site sequence. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab49822于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/500.
|
ICC/IF |
1/1000.
|
说明 |
---|
WB
1/500. |
ICC/IF
1/1000. |
靶标
-
功能
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. -
组织特异性
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. -
序列相似性
Belongs to the peptidase C14A family. -
翻译后修饰
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 836 Human
- Omim: 600636 Human
- SwissProt: P42574 Human
- Unigene: 141125 Human
-
别名
- A830040C14Rik antibody
- Apopain antibody
- CASP 3 antibody
see all
图片
-
Anti-Caspase-3 antibody (ab49822) at 1/1000 dilution
Secondary
Goat Anti-Rabbit IgG, Peroxidase conjugate
Developed using the ECL technique.
Observed band size: 17 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Cleaved Caspase-3 with ab49822 at 1/1000. HeLa cells were treated with 1 μg/mL staurosporine, fixed and permeabilized with Methanol followed by Acetone. Goat Anti-Rabbit IgG (whole molecule)-FITC was used as the secondary antibody. Cells were counterstained with DAPI (blue) to stain nuclei.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (388)
ab49822 被引用在 388 文献中.
- Song BW et al. Cold-pressed oil from Citrus aurantifolia inhibits the proliferation of vascular smooth muscle cells via regulation of PI3K/MAPK signaling pathways. Exp Ther Med 23:21 (2022). PubMed: 34815773
- Dai HT et al. microRNA-375 inhibits the malignant behaviors of hepatic carcinoma cells by targeting NCAPG2. Neoplasma 69:16-27 (2022). PubMed: 34818025
- Jiang J et al. Hyperoxia induces alveolar epithelial cell apoptosis by regulating mitochondrial function through small mothers against decapentaplegic 3 (SMAD3) and extracellular signal-regulated kinase 1/2 (ERK1/2). Bioengineered 13:242-252 (2022). PubMed: 34898379
- Wu Q et al. A Single Administration of Riluzole Applied Acutely After Spinal Cord Injury Attenuates Pro-inflammatory Activity and Improves Long-Term Functional Recovery in Rats. J Mol Neurosci 72:730-740 (2022). PubMed: 34988900
- Sahyon HAE et al. Anti-proliferative effects of the combination of Sulfamethoxazole and Quercetin via caspase3 and NFkB gene regulation: an in vitro and in vivo study. Naunyn Schmiedebergs Arch Pharmacol 395:227-246 (2022). PubMed: 34994822