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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin

Anti-Actin抗体- Loading Control (ab1801)

  • Datasheet
  • SDS
Reviews (11)Q&A (30)References (180)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody - Loading Control (ab1801)
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)

Key features and details

  • Rabbit polyclonal to Actin - Loading Control
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Actin抗体- Loading Control
    参阅全部 Actin 一抗
  • 描述

    兔多克隆抗体to Actin - Loading Control
  • 宿主

    Rabbit
  • 特异性

    This antibody recognises beta and gamma actin in Human samples. It probably also recognises all the other known forms of Human actin. This antibody detects a single clean band in Human, Mouse, Rat, Chicken and Drosophila samples. In Xenopus laevis a secondary band is detected at about 30kDa. We are unsure whether this is cross-reaction with another actin isoform or merely non-specific. In Cow a doublet is detected, which probably represents different forms of actin.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Human Actin aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab13771)

  • 阳性对照

    • HeLa whole cell lysate or mouse brain lysate. IHC-P - Human Colon FFPE tissue section

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Tropomyosin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • RIP
    • Isotype/Loading Controls
    • Loading Controls
    • Actin
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • RIP

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Actin protein (Tagged) (ab235861)
  • Related Products

    • Wiskostatin, N-WASP inhibitor (ab141085)
    • CK 666, Actin polymerization inhibitor (ab141231)
    • Jasplakinolide, Actin polymerization and stabilization inducer (ab141409)
    • Cytochalasin E, actin-polymerization inhibitor (ab141788)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Human
WB
Mouse
Rat
Human
All applications
Rabbit
Chicken
Cow
Saccharomyces cerevisiae
Xenopus laevis
Drosophila melanogaster
Zebrafish
Orangutan
应用 Ab评论 说明
WB (9)
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Block in 5% BSA. Blocking in milk significantly reduces the signal.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Block in 5% BSA. Blocking in milk significantly reduces the signal.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    • 功能

      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • 疾病相关

      Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • 序列相似性

      Belongs to the actin family.
    • 细胞定位

      Cytoplasm > cytoskeleton.
    • Target information above from: UniProt accession P68133 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 421534 Chicken
      • Entrez Gene: 281592 Cow
      • Entrez Gene: 58 Human
      • Entrez Gene: 11459 Mouse
      • Entrez Gene: 29437 Rat
      • Entrez Gene: 407658 Zebrafish
      • Omim: 102610 Human
      • SwissProt: P68139 Chicken
      • SwissProt: P68138 Cow
      • SwissProt: P68133 Human
      • SwissProt: P68134 Mouse
      • SwissProt: P68135 Rabbit
      • SwissProt: P68136 Rat
      • Unigene: 1288 Human
      • Unigene: 214950 Mouse
      • Unigene: 82732 Rat
      see all
    • 别名

      • a actin antibody
      • a-actin antibody
      • ACTA antibody
      • ACTA1 antibody
      • Actin alpha skeletal muscle antibody
      • Actin antibody
      • actin, alpha 1, skeletal muscle 1 antibody
      • actin, alpha 1, skeletal muscle antibody
      • Actin, alpha skeletal muscle antibody
      • actina antibody
      • actine antibody
      • ACTS_HUMAN antibody
      • aktin antibody
      • Alpha Actin 1 antibody
      • Alpha skeletal muscle Actin antibody
      • alpha skeletal muscle antibody
      • alpha-actin antibody
      • Alpha-actin-1 antibody
      • ASMA antibody
      • CFTD antibody
      • CFTD1 antibody
      • CFTDM antibody
      • MPFD antibody
      • NEM1 antibody
      • NEM2 antibody
      • NEM3 antibody
      • nemaline myopathy type 3 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody - Loading Control (ab1801)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody - Loading Control (ab1801)

      IHC image of ab1801 staining Actin in normal human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1801, 5μl/ml concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - Anti-Actin antibody - Loading Control (ab1801)
      Western blot - Anti-Actin antibody - Loading Control (ab1801)
      All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Brain (Rat) Tissue Lysate
      Lane 3 : Brain (Mouse) Tissue Lysate
      Lane 4 : NIH/3T3 whole cell lysate (ab7179)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa


      Exposure time: 1 minute


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab1801 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

    • Western blot - Anti-Actin antibody - Loading Control (ab1801)
      Western blot - Anti-Actin antibody - Loading Control (ab1801)Image from PLoS One. 2014; 9(3): e92128. Fig 9,•DOI: 10.1371/journal.pone.0092128 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Western blot analysis of HeLa cells treated for 12 hours with hesperidin (h) (2.5 μg/ml, 4,01 μM), mangiferin (5 μg/ml, 11.84 μM) (m), and hesperidin (2.5 μg/ml, 4.01 μM) in a presence of mangiferin (5 μg/ml, 11.84 μM) (h+m). Immunoblotting was performed with the following primary antibodies: Bax (ab32503), BCL2 (ab59348), beta actin (ab1801), and caspase 8. After the washing steps, the membranes were incubated with goat anti-rabbit IgG (H+L) or with goat anti-mouse IgG (H+L) HRP-conjugated secondary antibodies and detected using ECL. Densitometry was performed using Image Lab software v. 4.1 (BioRad).

      Top panel: Following 12h of treatment of HeLa cells with hesperidin (h), mangiferin (m), and hesperidin in a presence of mangiferin (h+m), the mRNA levels were monitored in real - time PCR experiments. The BAX and BCL2 mRNA levels results from 2 independent experiments (n?=?8) are plotted relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 18S rRNA levels and expressed as a fold change over the EtOH control. Error bars represent standard derivations.

      Bottom panel: Following 12h of treatment of HeLa cells with hesperidin (h), mangiferin (m), and hesperidin in a presence of mangiferin (h+m), the protein levels of Bax and BCL2 were detected with SDS-PAGE and Western Blot and related to beta actin levels.

    • Western blot - Anti-Actin antibody - Loading Control (ab1801)
      Western blot - Anti-Actin antibody - Loading Control (ab1801)
      All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate (ab27253)
      Lane 2 : NIH/3T3 (Mouse) Whole Cell Lysate (ab52956)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 42 kDa


      Exposure time: 30 seconds
    • Western blot - Anti-Actin antibody - Loading Control (ab1801)
      Western blot - Anti-Actin antibody - Loading Control (ab1801)This image is courtesy of an anonymous Abreview
      All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution

      All lanes : Whole cell lysates prepared from HUVEC cells

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat polyclonal to rabbit Ig at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 42 kDa


      Exposure time: 30 seconds

      See Abreview

    实验方案

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (180)

    发表研究结果有使用 ab1801?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1801 被引用在 180 文献中.

    • Qiao W  et al. miR-224-5p protects dental pulp stem cells from apoptosis by targeting Rac1. Exp Ther Med 19:9-18 (2020). PubMed: 31897093
    • Li Y & Chen Z The oncogenic role of microRNA-500a in colorectal cancer. Oncol Lett 19:1799-1805 (2020). PubMed: 32194673
    • Imamichi H  et al. Defective HIV-1 proviruses produce viral proteins. Proc Natl Acad Sci U S A 117:3704-3710 (2020). PubMed: 32029589
    • Korbut AI  et al. SGLT2 Inhibitor Empagliflozin and DPP4 Inhibitor Linagliptin Reactivate Glomerular Autophagy in db/db Mice, a Model of Type 2 Diabetes. Int J Mol Sci 21:N/A (2020). PubMed: 32340263
    • Yoshida S  et al. Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes. Nat Commun 11:2652 (2020). PubMed: 32461611
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 41 Abreviews or Q&A

    Western blot abreview for Anti-Actin antibody - Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (cancer cell lines)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    15 µg
    Specification
    cancer cell lines
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 03 2019

    Western blot abreview for Anti-Actin antibody - Loading Control

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (RIPA lysate of whole lung tissue)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    100 µg
    Specification
    RIPA lysate of whole lung tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Ben Thomson

    Verified customer

    提交于 Aug 14 2015

    Western blot abreview for Anti-Actin antibody - Loading Control

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (4-20% gradient gel)
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Specification
    HeLa cells
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 21 2014

    Immunocytochemistry/ Immunofluorescence

    Average
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Sample
    African Green Monkey Cell (COS7)
    Specification
    COS7
    Permeabilization
    Yes - Triton X 100 0.3%
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 03 2013

    Question


    Sorry it has taken me so long to respond to your email. We have tested the antibody in HeLa cell and no actin bands were detected. We have used these cells previously in other experiments, so we expect them to work with the Western.
    We would greatly appreciate a refund for the product (ab1801).
    Thank you,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 15 2012

    Answer

    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet. Since this order was paid for by credit card, the refund will go back onto the card. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Abcam Scientific Support

    回复于 Nov 15 2012

    Question

    We ordered anti-actin rabbit polyclonal antibody (ab1801, ordered 8/31/12) and have not been able to get the antibody
    to detect actin in a Western blot. We have followed the instructions for the antibody, which includes blocking in 5%
    BSA and using a 1/1000 dilution. We have tried not only BSA, but 5% milk (even though the instructions state that milk reduces the signal). We have also tried a 1/500 dilution for the antibody. We have checked that the protein has transferred well using PonceauS stain. We have tried the Western using different anti-actin antibody as a control and have been able to detect actin. We have tried the Western with ab1801 several times with no luck.
    Do you have other suggestions for the antibody? Would it be possible to get a refund or replacement for this antibody?
    It is quite frustrating to do a Western blot where the experiment seems to be working, but our results are inconclusive because our actin control is not working.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2012

    Answer

    Thank you for taking time to contact us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Could I just ask to confirm the species that your samples were from (i.e. mouse)?

    As long as the antibody was being used in a tested species, I would be happy to offer you a replacment or refund. Please let me know which you would prefer.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    回复于 Oct 25 2012

    Question

    I have used ab1801 to blot for actin from human whole cell extracts and seen no bands; stripping and reprobing with another anti-actin antibody provides expected results;

    Read More

    Abcam community

    Verified customer

    Asked on Aug 27 2012

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab8227.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Aug 27 2012

    Question

    Last month I order Anti-Actin antibody - Loading Control (ab1801) from your company.

    I ran the western blot assays with this antibody (rabbit, 1:1000) and used anti-rabbit secondary antibody (1:1000), but I get the white bands of actin (light black background)and dark black bands of molecular markersin the X-tay films. At the same time, I use the anti-tubulin (cell signaling) with the same procedures andget the normal balck bands oftubulinin the X-ray films.

    Could you tell me what is the problem?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 17 2012

    Answer

    Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

    It sounds like you're seeing "inverse banding" which is white bands on a black background. Usually this means that you're using too much primary and/or secondary antibody. I'd recommend diluting the primary to 1:2000 and incubating this overnight at 4C in the blocking buffer (5% BSA) and using the secondary at 1:5000 - 10,000 for 1 hr at RT in blocking buffer. A 1:1000 dilution for a secondary antibody is quite high.

    Are you blocking in 5% BSA?

    How much protein are you loading and what are your samples (species, cell line, etc.)?

    Do you have an image that you can send?


    I would strongly recommend that you try diluting the primary and secondary more. Should the suggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

    Read More

    Abcam Scientific Support

    回复于 Aug 17 2012

    Question


    Could you please provide us a testing code for ab121733?
    Thank you very much for all your support.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 14 2012

    Answer

    Thank you very much for your reply.

    Please see below for the testing code to try ab121733 with zebrafish samples:

    DISCOUNT CODE: ***
    EXPIRATION DATE: December 12, 2012

    I do hope that this antibody will work well with zebrafish, but if you need any assitance optimizing the protocol or anything else, please let me know and I'll be happy to help.

    Have a nice day!

    Read More

    Abcam Scientific Support

    回复于 Aug 14 2012

    Question


    Thank you for your reply. We are wondering if it is possible to use
    testing code for replacing anti-WDR81 primary antibody with another
    antibody (since there is no another Abcam WDR81 antibodies to compare it
    to). I am asking this in order to make sure the conditions of using
    testing code in case ab121733 does not react with zebrafish samples even
    with higher concentrations.
    Have a nice weekend.
    Best,

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    Abcam community

    Verified customer

    Asked on Aug 13 2012

    Answer

    Thank you very much for your reply.

    The testing codes can actuallybe used to receive any primary antibody in our catalog, regardless of target. So, if the WDR81 antibody does not work with zebrafish samples, you can use the code to get any other primary antibody of your choosing.

    I hope that this information will be useful, but please let me know if you have further questions. Have a nice day!

    Read More

    Abcam Scientific Support

    回复于 Aug 13 2012

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