重组Anti-ACSL1抗体[EPR13498] (ab178419)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13498] to ACSL1
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ACSL1抗体[EPR13498]
参阅全部 ACSL1 一抗 -
描述
兔单克隆抗体[EPR13498] to ACSL1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide within Human ACSL1 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: P33121 -
阳性对照
- Human fetal liver, heart and kidney lysates; HeLa and HepG2 cell lysates; Human heart, kidney and liver tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR13498 -
同种型
IgG -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178419于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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|
WB |
1/1000 - 1/5000. Predicted molecular weight: 78 kDa.
|
说明 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Predicted molecular weight: 78 kDa. |
靶标
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功能
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. -
组织特异性
Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas. -
序列相似性
Belongs to the ATP-dependent AMP-binding enzyme family. -
发展阶段
Expressed during the early stages of erythroid development while expression is very low in reticulocytes and young erythrocytes. -
细胞定位
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2180 Human
- Omim: 152425 Human
- SwissProt: P33121 Human
- Unigene: 406678 Human
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别名
- ACS 1 antibody
- ACS1 antibody
- ACSL 1 antibody
see all
图片
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All lanes : Anti-ACSL1 antibody [EPR13498] (ab178419) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ACSL1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDaLanes 1-3: Merged signal (red and green). Green - ab178419 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.
ab178419 Anti-ACSL1 antibody [EPR13498] was shown to specifically react with ACSL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265555 (knockout cell lysate ab257335) was used. Wild-type and ACSL1 knockout samples were subjected to SDS-PAGE. ab178419 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ACSL1 with ab178419 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-ACSL1 antibody [EPR13498] (ab178419) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 78 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ACSL1 with ab178419 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ACSL1 with ab178419 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab178419 被引用在 1 文献中.
- Belkaid A et al. 17ß-estradiol-induced ACSL4 protein expression promotes an invasive phenotype in estrogen receptor positive mammary carcinoma cells. Carcinogenesis 38:402-410 (2017). PubMed: 28334272