Aconitase Assay试剂盒(ab83459)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Tissue Lysate
概述
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产品名称
Aconitase Assay试剂盒
参阅全部 Aconitase 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell Lysate, Tissue Lysate -
检测类型
Enzyme activity -
检测时间
0h 40m -
产品概述
Aconitase Assay Kit ab83459 is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples.
In the aconitase assay protocol, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a max absorbance at 450nm.
Aconitase assay protocol summary:
- activate aconitase in samples by adding cysteine HCl and (NH4)2Fe(SO4)2
- add samples and standards to wells
- add reaction mix and incubate for 30-60 min
- analyze with a microplate reader -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K716 Aconitase Activity Colorimetric Assay Kit. K716-100 is the same size as the 100 test size of ab83459.
Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process.
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平台
Microplate reader
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 100 tests Ferrous Ammonium Sulfate 1 vial Assay Buffer VII 1 x 25ml Cysteine Standard 1 vial Developer Solution II 1 vial Enzyme Mix VII 1 x 600µl Isocitrate Standard 1 x 100µl Substrate XI 1 vial -
研究领域
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相关性
Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with prooxidants has been interpreted as a measure of oxidative damage. -
细胞定位
ACO1: Cytoplasmic ACO2: Mitochondrial -
别名
- ACO 1
- ACO 2
- ACO1
see all
图片
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Aconitase measured in mouse tissue lysates showing quantity (mU) per mg of tested sample.
Protein concentration for samples varied from 1.5 mg/mL to 10 mg/mL. Samples were diluted 1-27 fold.
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Mitochondrial autophagic profiles in ON vulnerable to secondary degeneration +/- R/NIR-IT. Accumulations of mitochondrial autophagic profiles (red arrows) were seen especially at day 1 after PT (A, B). Occasionally, larger features reminiscent of autophagosomes were observed (A, black arrow) and parts of the nerve appeared highly abnormal (*, B).
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30 minute Aconitase sample test using ab83459.
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Isocitrate standard curve using ab83459.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab83459 被引用在 15 文献中.
- Álvarez-Córdoba M et al. Down regulation of the expression of mitochondrial phosphopantetheinyl-proteins in pantothenate kinase-associated neurodegeneration: pathophysiological consequences and therapeutic perspectives. Orphanet J Rare Dis 16:201 (2021). PubMed: 33952316
- Yao F et al. Iron regulatory protein 1 promotes ferroptosis by sustaining cellular iron homeostasis in melanoma. Oncol Lett 22:657 (2021). PubMed: 34386079
- Ghaisas S et al. Chronic Manganese Exposure and the Enteric Nervous System: An in Vitro and Mouse in Vivo Study. Environ Health Perspect 129:87005 (2021). PubMed: 34410835
- Flockhart M et al. Excessive exercise training causes mitochondrial functional impairment and decreases glucose tolerance in healthy volunteers. Cell Metab 33:957-970.e6 (2021). PubMed: 33740420
- Lombardi M et al. Mitochondrial Energetics and Ca2+-Activated ATPase in Obstructive Hypertrophic Cardiomyopathy. J Clin Med 9:N/A (2020). PubMed: 32527005