Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
50 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5 %
Other product details
Dilution
1/200
Incubation time
14 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 250, 90, 75 kDa Non-specific: 130, 50 kDa
Positive control
Immunoprecipitation of RNPS1 and SAP18, two proteins known to interact with Acinus (see lanes 5 and 6)
Negative control
FLAG-alone immunoprecipitation (lane 4)
Additional data
Abcam response
Thank you for your feedback.
Diluting out the primary more and using less protein per lane may reduce some of the non specific bands. Use of a blocking peptide( ab19644) with this antibody may give more information about which bands were specific as this antibody has not been tested in IP yet.
Diluting out the primary more and using less protein per lane may reduce some of the non specific bands. Use of a blocking peptide( ab19644) with this antibody may give more information about which bands were specific as this antibody has not been tested in IP yet.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 05 2007