重组Anti-Acid sphingomyelinase抗体[EPR23090-181] (ab272729)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23090-181] to Acid sphingomyelinase
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Acid sphingomyelinase抗体[EPR23090-181]
参阅全部 Acid sphingomyelinase 一抗 -
描述
兔单克隆抗体[EPR23090-181] to Acid sphingomyelinase -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: THP-1 treated with 80nM phorbol-12-myristate-13-acetate and HepG2 whole cell lysates; Human heart tissue lysate.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23090-181 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272729于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa).
|
说明 |
---|
WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa). |
靶标
-
功能
Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. -
疾病相关
Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPDA) [MIM:257200]; also known as Niemann-Pick disease classical infantile form. It is an early-onset lysosomal storage disorder caused by failure to hydrolyze sphingomyelin to ceramide. It results in the accumulation of sphingomyelin and other metabolically related lipids in reticuloendothelial and other cell types throughout the body, leading to cell death. Niemann-Pick disease type A is a primarily neurodegenerative disorder characterized by onset within the first year of life, mental retardation, digestive disorders, failure to thrive, major hepatosplenomegaly, and severe neurologic symptoms. The severe neurological disorders and pulmonary infections lead to an early death, often around the age of four. Clinical features are variable. A phenotypic continuum exists between type A (basic neurovisceral) and type B (purely visceral) forms of Niemann-Pick disease, and the intermediate types encompass a cluster of variants combining clinical features of both types A and B.
Defects in SMPD1 are the cause of Niemann-Pick disease type B (NPDB) [MIM:607616]; also known as Niemann-Pick disease visceral form. It is a late-onset lysosomal storage disorder caused by failure to hydrolyze sphingomyelin to ceramide. It results in the accumulation of sphingomyelin and other metabolically related lipids in reticuloendothelial and other cell types throughout the body, leading to cell death. Clinical signs involve only visceral organs. The most constant sign is hepatosplenomegaly which can be associated with pulmonary symptoms. Patients remain free of neurologic manifestations. However, a phenotypic continuum exists between type A (basic neurovisceral) and type B (purely visceral) forms of Niemann-Pick disease, and the intermediate types encompass a cluster of variants combining clinical features of both types A and B. In Niemann-Pick disease type B, onset of the first symptoms occurs in early childhood and patients can survive into adulthood. -
序列相似性
Belongs to the acid sphingomyelinase family.
Contains 1 saposin B-type domain. -
细胞定位
Lysosome. - Information by UniProt
-
数据库链接
- Entrez Gene: 6609 Human
- Omim: 607608 Human
- SwissProt: P17405 Human
- Unigene: 498173 Human
-
别名
- Acid sphingomyelinase antibody
- ASM antibody
- ASM_HUMAN antibody
see all
图片
-
Anti-Acid sphingomyelinase antibody [EPR23090-181] (ab272729) at 1/500 dilution + Human heart tissue lysate at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration.This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes.
-
All lanes : Anti-Acid sphingomyelinase antibody [EPR23090-181] (ab272729) at 1/1000 dilution
Lane 1 : Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 2 : THP-1 treated with 80nM phorbol-12-myristate-13-acetate (PMA) (ab120297) overnight whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 70 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 25803076, 25853898).
ASM expression can be induced by PMA in THP-1 cells (PMID:10224156).
Exposure time: 3 minutes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (2)
ab272729 被引用在 2 文献中.
- Du YX et al. Acid sphingomyelinase mediates ferroptosis induced by high glucose via autophagic degradation of GPX4 in type 2 diabetic osteoporosis. Mol Med 29:125 (2023). PubMed: 37710183
- Momchilova A et al. Sphingolipid Catabolism and Glycerophospholipid Levels Are Altered in Erythrocytes and Plasma from Multiple Sclerosis Patients. Int J Mol Sci 23:N/A (2022). PubMed: 35886939