Anti-Acetylcholinesterase抗体[HR2] (ab2803)
Key features and details
- Mouse monoclonal [HR2] to Acetylcholinesterase
- Suitable for: Flow Cyt, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2b
概述
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产品名称
Anti-Acetylcholinesterase抗体[HR2]
参阅全部 Acetylcholinesterase 一抗 -
描述
小鼠单克隆抗体[HR2] to Acetylcholinesterase -
宿主
Mouse -
特异性
This antibody does not detect butyrylcholinesterase (BChE). -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF MouseHumanIHC-P Human -
免疫原
Full length protein corresponding to Human Acetylcholinesterase. Purified Human cerebellar acetylcholinesterase.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
HR2 -
同种型
IgG2b -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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Flow Cyt |
Human
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ICC/IF |
Mouse
Human
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IHC-P |
Human
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All applications |
Non human primates
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应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. -
组织特异性
Isoform H is highly expressed in erythrocytes. -
序列相似性
Belongs to the type-B carboxylesterase/lipase family. -
细胞定位
Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei. - Information by UniProt
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数据库链接
- Entrez Gene: 43 Human
- Entrez Gene: 11423 Mouse
- Omim: 100740 Human
- SwissProt: P22303 Human
- SwissProt: P21836 Mouse
- Unigene: 154495 Human
- Unigene: 255464 Mouse
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别名
- ACEE antibody
- ACES_HUMAN antibody
- Acetylcholinesterase antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Acetylcholinesterase (ab2803) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunocytochemistry/Immunofluorescence analysis of Acetylcholinesterase shows staining in HeLa cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2803 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunocytochemistry/Immunofluorescence analysis of Acetylcholinesterase shows staining in Neuro-2a cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2803 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunocytochemistry/Immunofluorescence analysis of Acetylcholinesterase shows staining in U251 cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2803 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Cerebellum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Acetylcholinesterase (ab2803) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Rectum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Acetylcholinesterase (ab2803) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Overlay histogram showing HeLa cells stained with ab2803 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2803, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab2803 被引用在 6 文献中.
- Dafferner AJ et al. Immunopurification of Acetylcholinesterase from Red Blood Cells for Detection of Nerve Agent Exposure. Chem Res Toxicol 30:1897-1910 (2017). PubMed: 28892361
- Yu ZG et al. Effects of Zusanli and Ashi Acupoint Electroacupuncture on Repair of Skeletal Muscle and Neuromuscular Junction in a Rabbit Gastrocnemius Contusion Model. Evid Based Complement Alternat Med 2016:7074563 (2016). PubMed: 27190536
- Smith RS et al. Differential Muscarinic Modulation in the Olfactory Bulb. J Neurosci 35:10773-85 (2015). IHC-FrFl ; Mouse . PubMed: 26224860
- Zitella LM et al. In Vivo 7T MRI of the Non-Human Primate Brainstem. PLoS One 10:e0127049 (2015). IHC . PubMed: 25965401
- Lavenex P et al. Postmortem changes in the neuroanatomical characteristics of the primate brain: hippocampal formation. J Comp Neurol 512:27-51 (2009). IHC-FrFl ; Macaque monkey . PubMed: 18972553
- Rakonczay Z & Brimijoin S Monoclonal antibodies to human brain acetylcholinesterase: properties and applications. Cell Mol Neurobiol 8:85-93 (1988). PubMed: 3401901