重组Anti-Acetyl Coenzyme A Carboxylase抗体[EP687Y] (ab45174)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP687Y] to Acetyl Coenzyme A Carboxylase
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Anti-Acetyl Coenzyme A Carboxylase抗体[EP687Y]
参阅全部 Acetyl Coenzyme A Carboxylase 一抗 -
描述
兔单克隆抗体[EP687Y] to Acetyl Coenzyme A Carboxylase -
宿主
Rabbit -
特异性
Acetyl Coenzyme A Carboxylase is highly expressed in lipogenic tissues such as liver, adipose, and lactating mammary gland, and its activities are regulated at various levels [Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7515-20.]. -
Tested Applications & Species
Application Species IHC-P MouseRatHumanWB MouseRatHuman -
免疫原
Synthetic peptide within Human Acetyl Coenzyme A Carboxylase aa 350-450. The exact sequence is proprietary.
(Peptide available asab195232) -
阳性对照
- WB: HAP1, HeLa, C2C12 and A431 cell lysate; Rat adrenal gland tissue lysates IHC-P: Human liver and breast carcinoma tissue, Rat stomcah tissue, Mouse kidney tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP687Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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IHC-P |
Mouse
Rat
Human
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WB |
Mouse
Rat
Human
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应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/1000 - 1/2000. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).Can be blocked with Acetyl Coenzyme A Carboxylase peptide (ab195232).
|
IHC-P |
1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
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WB
1/1000 - 1/2000. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).Can be blocked with Acetyl Coenzyme A Carboxylase peptide (ab195232). |
IHC-P
1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. -
组织特异性
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. -
通路
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. -
疾病相关
Acetyl-CoA carboxylase 1 deficiency -
序列相似性
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
翻译后修饰
Phosphorylation on Ser-1263 is required for interaction with BRCA1. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 31 Human
- Entrez Gene: 32 Human
- Entrez Gene: 100705 Mouse
- Entrez Gene: 107476 Mouse
- Entrez Gene: 116719 Rat
- Entrez Gene: 60581 Rat
- Omim: 200350 Human
- Omim: 601557 Human
see all -
别名
- ACAC antibody
- ACACA antibody
- ACACA_HUMAN antibody
see all
图片
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All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174) at 1/2000 dilution (unpurified)
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ACACA (Acetyl Coenzyme A Carboxylase) knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 265 kDaLanes 1 - 4: Merged signal (red and green). Green - ab45174 observed at 265 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab45174 was shown to specifically react with Acetyl Coenzyme A Carboxylase in wild-type HAP1 cells as signal was lost in ACACA (Acetyl Coenzyme A Carboxylase) knockout cells. Wild-type and ACACA (Acetyl Coenzyme A Carboxylase) knockout samples were subjected to SDS-PAGE. Ab45174 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling Acetyl Coenzyme A Carboxylase with purified ab45174 at 1/400 dilution (2.06 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174) at 1/1000 dilution (Purified)
Lane 1 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 2 : Rat adrenal gland lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 265 kDa
Observed band size: 265 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
We are unsure how to define the extra bands.
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Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174) at 1/1000 dilution (Purified) + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 265 kDa
Observed band size: 265 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomcah tissue sections labeling Acetyl Coenzyme A Carboxylase with purified ab45174 at 1/400 dilution (2.06 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Acetyl Coenzyme A Carboxylase with purified ab45174 at 1/400 dilution (2.06 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
ab45174 (unpurified), at a dilution of 1/50, staining human Acetyl Coenzyme A Carboxylase in human liver by immunohistochemistry using paraffin embedded tissue. Heat mediated antigen retrieval was perfromed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Acetyl Coenzyme A Carboxylase with ab45174 (unpurified) at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab45174 被引用在 23 文献中.
- Li M et al. Sirt3 modulates fatty acid oxidation and attenuates cisplatin-induced AKI in mice. J Cell Mol Med 24:5109-5121 (2020). PubMed: 32281286
- Zhou Y et al. PMP22 Regulates Cholesterol Trafficking and ABCA1-Mediated Cholesterol Efflux. J Neurosci 39:5404-5418 (2019). PubMed: 31061090
- Chen XY et al. LB100 ameliorates nonalcoholic fatty liver disease via the AMPK/Sirt1 pathway. World J Gastroenterol 25:6607-6618 (2019). PubMed: 31832001
- Jia H et al. Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly. J Dairy Sci 102:833-845 (2019). PubMed: 30415861
- Chen Y et al. Long noncoding RNA FLRL2 alleviated nonalcoholic fatty liver disease through Arntl-Sirt1 pathway. FASEB J 33:11411-11419 (2019). PubMed: 31311301