Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Alveolar Epithelial Cell)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Human Alveolar Epithelial Cell
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% Donkey serum in PBST
Dilution
1/15
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-Rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Red - ACE2 blue - nucleus, DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Armen Petrosyan
Verified customer
提交于 Jul 02 2020