Anti-ACADVL/VLCAD抗体(ab155138)
Key features and details
- Rabbit polyclonal to ACADVL/VLCAD
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-ACADVL/VLCAD抗体
参阅全部 ACADVL/VLCAD 一抗 -
描述
兔多克隆抗体to ACADVL/VLCAD -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Recombinant fragment corresponding to Human ACADVL/VLCAD aa 18-341.
Database link: P49748 -
阳性对照
- H1299, HeLa, HepG2 and mouse liver whole cell lysates, HeLa cells, H358 xenograft
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155138于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/100 - 1/1000.
|
说明 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
靶标
-
功能
Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accomodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons. -
通路
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
疾病相关
Defects in ACADVL are the cause of acyl-CoA dehydrogenase very long chain deficiency (ACADVLD) [MIM:201475]. ACADVLD is an autosomal recessive disease which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form, with early onset, high mortality, and high incidence of cardiomyopathy; a milder childhood form, with later onset, usually with hypoketotic hypoglycemia as the main presenting feature, low mortality, and rare cardiomyopathy; and an adult form, with isolated skeletal muscle involvement, rhabdomyolysis, and myoglobinuria, usually triggered by exercise or fasting. -
序列相似性
Belongs to the acyl-CoA dehydrogenase family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 37 Human
- Entrez Gene: 11370 Mouse
- Entrez Gene: 25363 Rat
- Omim: 609575 Human
- SwissProt: P49748 Human
- SwissProt: P50544 Mouse
- SwissProt: P45953 Rat
- Unigene: 437178 Human
see all -
别名
- ACAD 6 antibody
- ACAD6 antibody
- ACADV_HUMAN antibody
see all
图片
-
Anti-ACADVL/VLCAD antibody (ab155138) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 70 kDa
7.5% SDS PAGE -
Anti-ACADVL/VLCAD antibody (ab155138) at 1/3000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACADVL/VLCAD with ab155138 at 1/200. Image at the bottom costained with Hoechst 33342.
-
Immunohistochemical analysis of paraffin embedded H358 xenograft labeling ACADVL/VLCAD with ab155138 antibody at 1/250.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (11)
ab155138 被引用在 11 文献中.
- Peng MZ et al. Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism. Cell Mol Life Sci 79:375 (2022). PubMed: 35727412
- Huang CY et al. PERM1 regulates genes involved in fatty acid metabolism in the heart by interacting with PPARα and PGC-1α. Sci Rep 12:14576 (2022). PubMed: 36028747
- Svensson K et al. Combined overexpression of SIRT1 and knockout of GCN5 in adult skeletal muscle does not affect glucose homeostasis or exercise performance in mice. Am J Physiol Endocrinol Metab 318:E145-E151 (2020). WB ; Mouse . PubMed: 31794263
- Jestin M et al. Mitochondrial disease disrupts hepatic allostasis and lowers the threshold for immune-mediated liver toxicity. Mol Metab 37:100981 (2020). PubMed: 32283081
- Zhou X et al. Brown adipose tissue-derived exosomes mitigate the metabolic syndrome in high fat diet mice. Theranostics 10:8197-8210 (2020). PubMed: 32724466