Anti-ACAA1/Beta-ketothiolase抗体(ab154091)
Key features and details
- Rabbit polyclonal to ACAA1/Beta-ketothiolase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ACAA1/Beta-ketothiolase抗体
参阅全部 ACAA1/Beta-ketothiolase 一抗 -
描述
兔多克隆抗体to ACAA1/Beta-ketothiolase -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow -
免疫原
Recombinant fragment corresponding to Human ACAA1/Beta-ketothiolase aa 50-350.
Database link: P09110 -
阳性对照
- WB: A549, HeLa and mouse liver whole cell lysates; ICC/IF: HeLa cells; IHC-P: Mouse stomach;
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab154091于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
靶标
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通路
Lipid metabolism; fatty acid metabolism. -
序列相似性
Belongs to the thiolase family. -
细胞定位
Peroxisome. - Information by UniProt
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数据库链接
- Entrez Gene: 508324 Cow
- Entrez Gene: 30 Human
- Entrez Gene: 113868 Mouse
- Omim: 604054 Human
- SwissProt: P09110 Human
- SwissProt: Q921H8 Mouse
- Unigene: 643487 Human
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别名
- 3 ketoacyl CoA thiolase peroxisomal antibody
- 3-ketoacyl-CoA thiolase antibody
- ACAA antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse stomach staining ACAA1/Beta-ketothiolase with ab154091 at a dilution of 1/500.
EDTA based Antigen Retrieval, pH 8.0 for 15min.
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Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACAA1/Beta-ketothiolase with ab154091 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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All lanes : Anti-ACAA1/Beta-ketothiolase antibody (ab154091) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
10% SDS PAGE -
Anti-ACAA1/Beta-ketothiolase antibody (ab154091) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACAA1/Beta-ketothiolase with ab154091 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab154091 被引用在 1 文献中.
- Colasuonno F et al. Mitochondrial and Peroxisomal Alterations Contribute to Energy Dysmetabolism in Riboflavin Transporter Deficiency. Oxid Med Cell Longev 2020:6821247 (2020). PubMed: 32855765