Key features and details
- Rabbit polyclonal to ABLIM3
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
参阅全部 ABLIM3 一抗
经测试应用适用于: WBmore details
预测可用于: Rat, Human, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human ABLIM3 aa 550-650 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Mouse Embryonic Brain E16; Mouse Olfactory Bulb; Mouse Hippocampus; Mouse Cerebellum.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab129480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). Abcam recommends using milk as the blocking agent - 3%|
功能May act as scaffold protein. May stimulate ABRA activity and ABRA-dependent SRF transcriptional activity.
组织特异性Expressed predominantly in heart and brain.
序列相似性Contains 1 HP (headpiece) domain.
Contains 4 LIM zinc-binding domains.
- Information by UniProt
- abLIM-3 antibody
- Ablim3 antibody
- ABLM3_HUMAN antibody
All lanes : Anti-ABLIM3 antibody (ab129480) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : E16 Ms Embryo Brain Tissue Lysate
Lane 2 : Mouse Olfactory Bulb Lysate
Lane 3 : Mouse Hippocampus Tissue Lysate
Lane 4 : Cerebellum Mouse Tissue Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Additional bands at: 68 kDa, 70 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends using milk as the blocking agent - 3%. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab129480 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab129480 被引用在 1 文献中.
- Guo N et al. Dentate granule cell recruitment of feedforward inhibition governs engram maintenance and remote memory generalization. Nat Med 24:438-449 (2018). PubMed: 29529016