Anti-ABCG8抗体(ab223056)
Key features and details
- Rabbit polyclonal to ABCG8
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ABCG8抗体
参阅全部 ABCG8 一抗 -
描述
兔多克隆抗体to ABCG8 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human ABCG8 aa 176-413.
Database link: Q9H221 -
阳性对照
- WB: Mouse heart, lung, kidney and stomach lysates. IHC-P: Human lung tissue. ICC/IF: MCF7 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purity >95% -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223056于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
靶标
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功能
Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. -
组织特异性
Strongly expressed in the liver, lower levels in the small intestine and colon. Detectable in a wide variety of human tissues. -
疾病相关
Genetic variations in ABCG8 can be associated with susceptibility to gallbladder disease type 4 (GBD4) [MIM:611465]. With an overall prevalence of 10-20%, gallstone disease (cholelithiasis) represents one of the most frequent and economically relevant health problems of industrialized countries.
Defects in ABCG8 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease. -
序列相似性
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 64241 Human
- Entrez Gene: 67470 Mouse
- Omim: 605460 Human
- SwissProt: Q9H221 Human
- SwissProt: Q9DBM0 Mouse
- Unigene: 413931 Human
- Unigene: 26581 Mouse
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别名
- ABCG8 antibody
- ABCG8_HUMAN antibody
- ATP binding cassette sub family G (WHITE) member 8 (sterolin 2) antibody
see all
图片
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All lanes : Anti-ABCG8 antibody (ab223056) at 1/2000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse lung lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse stomach lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDa -
Paraffin-embedded human lung tissue stained for ABCG8 using ab223056 at 1/100 dilution in immunohistochemical analysis.
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MCF7 (human breast adenocarcinoma cell line) cells stained for ABCG8 (green) using ab223056 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG(H+L).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab223056 被引用在 2 文献中.
- Zhang Z et al. HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARa axis. Mol Ther Nucleic Acids 24:711-727 (2021). PubMed: 33996254
- Huang X et al. [Lipid-lowering effect of propolis in mice with Triton-WR1339-induced hyperlipidemia and its mechanism for regulating lipid metabolism]. Nan Fang Yi Ke Da Xue Xue Bao 38:1020-1024 (2018). PubMed: 30187881