概述

  • 产品名称
  • 描述
    兔多克隆抗体to ABCG8
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen, corresponding corresponding to amino acids 299-415 of Human ABCG8.

  • 阳性对照
    • Human duodenum tissue; RT 4 and U251 MG cell lysate, Human Plasma lysate

应用

Our Abpromise guarantee covers the use of ab126493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500. Predicted molecular weight: 76 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.
  • 组织特异性
    Strongly expressed in the liver, lower levels in the small intestine and colon. Detectable in a wide variety of human tissues.
  • 疾病相关
    Genetic variations in ABCG8 can be associated with susceptibility to gallbladder disease type 4 (GBD4) [MIM:611465]. With an overall prevalence of 10-20%, gallstone disease (cholelithiasis) represents one of the most frequent and economically relevant health problems of industrialized countries.
    Defects in ABCG8 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease.
  • 序列相似性
    Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABCG8 antibody
    • ABCG8_HUMAN antibody
    • ATP binding cassette sub family G (WHITE) member 8 (sterolin 2) antibody
    • ATP binding cassette sub family G member 8 antibody
    • ATP binding cassette, sub family G (WHITE), member 8 antibody
    • ATP-binding cassette sub-family G member 8 antibody
    • GBD4 antibody
    • MGC142217 antibody
    • Sterolin 2 antibody
    • Sterolin-2 antibody
    • STSL antibody
    see all

图片

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab126493 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab126493 at 1/50 dilution staining ABCG8 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
  • All lanes : Anti-ABCG8 antibody (ab126493) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique.

    Predicted band size: 76 kDa

文献

This product has been referenced in:
  • Castella B  et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and V?9Vd2 T cell activation by dendritic cells. Nat Commun 8:15663 (2017). Read more (PubMed: 28580927) »
  • Berger CN  et al. Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo. Cell Metab 26:738-752.e6 (2017). WB . Read more (PubMed: 28988824) »
See all 2 Publications for this product

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