山羊抗小鼠IgM mu chain (HRP) (ab97230)
Key features and details
- Goat Anti-Mouse IgM mu chain (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, WB, ICC, IHC-P
Related conjugates and formulations
概述
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产品名称
山羊抗小鼠IgM mu chain (HRP)
参阅全部 IgM 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgM. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. -
经测试应用
适用于: ELISA, WB, ICC, IHC-Pmore details -
偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
共轭说明
Molar enzyme/ antibody protein ratio is 4:1 -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97230于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
1/10000 - 1/100000. (Primary ELISA)
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WB |
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminiscence: 1/5000 - 1/25000)
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/2000.
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说明 |
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ELISA
1/10000 - 1/100000. (Primary ELISA) |
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminiscence: 1/5000 - 1/25000) |
ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
图片
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IHC image of Hsp70 staining in human lung formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab5442, 1/2000 dilution overnight at +4°C. An HRP-conjugated secondary (ab97230, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (48)
ab97230 被引用在 48 文献中.
- Stoler-Barak L et al. B cell class switch recombination is regulated by DYRK1A through MSH6 phosphorylation. Nat Commun 14:1462 (2023). PubMed: 36927854
- Affonso de Oliveira JF et al. COVID-19 vaccines based on viral nanoparticles displaying a conserved B-cell epitope show potent immunogenicity and a long-lasting antibody response. Front Microbiol 14:1117494 (2023). PubMed: 37152732
- Silva-Cayetano A et al. Spatial dysregulation of T follicular helper cells impairs vaccine responses in aging. Nat Immunol 24:1124-1137 (2023). PubMed: 37217705
- Liu Y et al. O-GlcNAcylation promotes topoisomerase IIα catalytic activity in breast cancer chemoresistance. EMBO Rep 24:e56458 (2023). PubMed: 37249035
- Liu Y et al. FOXA1 O-GlcNAcylation-mediated transcriptional switch governs metastasis capacity in breast cancer. Sci Adv 9:eadg7112 (2023). PubMed: 37595040