Anti-Interferon gamma抗体(ab25101)
Key features and details
- Rabbit polyclonal to Interferon gamma
- Suitable for: Sandwich ELISA, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Interferon gamma抗体
参阅全部 Interferon gamma 一抗 -
描述
兔多克隆抗体to Interferon gamma -
宿主
Rabbit -
特异性
This antibody neutralizes both natural and recombinant human IFN gamma. Activity =10^4 Neutralizing U/mg protein. -
经测试应用
适用于: Sandwich ELISA, IHC-Pmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human Interferon gamma.
Database link: P01579 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Constituents: 4.275% Trehalose, 95.725% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was sequentially purified by ammonium sulphate precipitation and protein affinity chromatography. The antibody was then membrane filtered (0.2 µm) for sterility. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Conjugation kits
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Isotype control
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Recombinant Protein
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25101于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MD-1] to Interferon gamma (ab25014).
For sandwich ELISA, use this antibody as detection at 0.5µg/ml with ab25014 as capture. |
|
IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MD-1] to Interferon gamma (ab25014). For sandwich ELISA, use this antibody as detection at 0.5µg/ml with ab25014 as capture. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
组织特异性
Released primarily from activated T lymphocytes. -
疾病相关
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
序列相似性
Belongs to the type II (or gamma) interferon family. -
翻译后修饰
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
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别名
- IF 1 antibody
- IFG antibody
- IFI antibody
see all
图片
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ab25101 staining Human tonsil. Staining is localised to the cytoplasm and protein is secreted.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab25101 被引用在 19 文献中.
- Xia P et al. NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis. BMC Immunol 23:39 (2022). PubMed: 35965334
- Ma L et al. Inflammation Mediates the Development of Aggressive Breast Cancer Following Radiotherapy. Clin Cancer Res 27:1778-1791 (2021). PubMed: 33402361
- Wu B et al. Mitochondrial aspartate regulates TNF biogenesis and autoimmune tissue inflammation. Nat Immunol 22:1551-1562 (2021). PubMed: 34811544
- Tummala P et al. Glutathione transferase Omega 1 confers protection against azoxymethane-induced colorectal tumour formation. Carcinogenesis 42:853-863 (2021). PubMed: 33564842
- Bahrami-Asl Z et al. Tacrolimus Improves the Implantation Rate in Patients with Elevated Th1/2 Helper Cell Ratio and Repeated Implantation Failure (RIF). Geburtshilfe Frauenheilkd 80:851-862 (2020). PubMed: 32817992