Anti-CD9抗体(ab223052)
Key features and details
- Rabbit polyclonal to CD9
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CD9抗体
参阅全部 CD9 一抗 -
描述
兔多克隆抗体to CD9 -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. We recommend ab307085 as an alternative for IHC-P.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: African green monkey -
免疫原
Recombinant fragment corresponding to Human CD9 aa 100-200.
Database link: P21926 -
阳性对照
- WB: Mouse lung and kidney tissue lysates. Rat lung and kidney tissue lysates. Hela whole cell lysate, HepG2 whole cell lysate, LO2 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, IHC-P: Human placenta, breast cancer, colon cancer, tonsil, bladder, Prostate gland cancer tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
>95% pure -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223052于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25 kDa).
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IHC-P |
1/30 - 1/100.
We do not guarantee IHC-P for mouse and rat. We recommend ab307085 as an alternative for IHC-P. |
说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25 kDa). |
IHC-P
1/30 - 1/100. We do not guarantee IHC-P for mouse and rat. We recommend ab307085 as an alternative for IHC-P. |
靶标
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功能
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. -
组织特异性
Expressed by a variety of hematopoietic and epithelial cells. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
翻译后修饰
Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 928 Human
- Entrez Gene: 12527 Mouse
- Omim: 143030 Human
- SwissProt: P30409 African green monkey
- SwissProt: P21926 Human
- SwissProt: P40240 Mouse
- Unigene: 114286 Human
- Unigene: 210676 Mouse
see all -
别名
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
see all
图片
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All lanes : Anti-CD9 antibody (ab223052) at 1/500 dilution
Lane 1 : Rat Liver tissue lysate at 50 µg
Lane 2 : Rat Lung tissue lysate at 20 µg
Lane 3 : Mouse Liver tissue lysate at 20 µg
Lane 4 : Mouse Lung tissue lysate at 20 µg
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
Left: IHC image of ab223052 diluted at 1:100 and staining in paraffin-embedded human tonsils tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Right: Negative Control
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Paraffin embedded sections of human placenta tissue were stained for CD9 with ab223052 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-CD9 antibody (ab223052) at 1/1000 dilution
Lane 1 : Hela whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : LO2 whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : U87 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
All lanes : Anti-CD9 antibody (ab223052) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate at 20 µg
Lane 2 : HepG2 whole cell lysate at 40 µg
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
Left: IHC image of ab223052 diluted at 1:100 and staining in paraffin-embedded human Prostate gland Cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Right: negative control
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Paraffin embedded sections of human colon cancer tissue were stained for CD9 with ab223052 at 1/100 dilution in immunohistochemical analysis.
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Left: IHC image of ab223052 diluted at 1:100 and staining in paraffin-embedded human bladder tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Right: Negative Control
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (40)
ab223052 被引用在 40 文献中.
- Lai C et al. Synovial fibroblast-miR-214-3p-derived exosomes inhibit inflammation and degeneration of cartilage tissues of osteoarthritis rats. Mol Cell Biochem 478:637-649 (2023). PubMed: 36001206
- Ye Q et al. Association between lncRNAs in plasma exosomes and diabetic retinopathy. Front Endocrinol (Lausanne) 13:987488 (2022). PubMed: 36187126
- Huang H et al. Identification of the Level of Exosomal Protein by Parallel Reaction Monitoring Technology in HCC Patients. Int J Gen Med 15:7831-7842 (2022). PubMed: 36267426
- Zhu C et al. Tumor-derived extracellular vesicles inhibit HGF/c-Met and EGF/EGFR pathways to accelerate the radiosensitivity of nasopharyngeal carcinoma cells via microRNA-142-5p delivery. Cell Death Discov 8:17 (2022). PubMed: 35013115
- Wang CK et al. Internalization and trafficking of CSPG-bound recombinant VAR2CSA lectins in cancer cells. Sci Rep 12:3075 (2022). PubMed: 35197518