Glycolysis Assay [Extracellular acidification] (ab197244)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Adherent cells, Suspension cells
概述
-
产品名称
Glycolysis Assay [Extracellular acidification] -
检测方法
Fluorescent -
样品类型
Adherent cells, Suspension cells -
检测类型
Cell-based (quantitative) -
检测时间
1h 30m -
产品概述
Glycolysis Assay [Extracellular Acidification] (ab197244) is an easy mix-and-measure, 96 well fluorescence plate reader-based approach for the analysis of extracellular acidification (ECA/ECAR). As lactate production is the main contributor to this acidification, ab197244 is a convenient and informative measure of cellular glycolytic flux. Such measurements offer an important insight into the central role played by altered glycolytic activity in a wide array of physiological and pathophysiological processes, including cellular adaptation to hypoxia and ischemia, and the development and progression of tumorigenesis.
The performance of Glycolysis Assay facilitates sensitive robust microtiter-plate based measurements, thereby overcoming many of the problems associated with the more cumbersome potentiometric pH approach. Rates of extracellular acidification are calculated from changes in fluorescence signal over time and, as the measurement is non-destructive and fully reversible (pH-sensitive reagent is not consumed), measurement of time-courses and multiple drug treatments are possible.
-
说明
Learn more about the full range of assays to measure glycolysis, oxygen consumption, fatty acid oxidation and metabolic flux in live cells.
Or review the full metabolism assay guide for other assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress.
-
平台
Microplate reader
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 96 tests 4 x 96 tests Glycolysis Assay Reagent (lyophilized) 1 vial 4 vials Respiration Buffer 1 tablet 4 tablets -
研究领域
相关产品
-
Related Products
图片
-
Cellular Energy Flux for HepG2 cells (seeded at 65,000 per well), treated with a combination of drug compounds modulating the ETC (Antimycin A [1 µM] and FCCP [2.5 µM]), shown as a percentage relative to untreated control cells. Comparative measurements were taken with Extracellular Oxygen Consumption Assay (ab197243) (white column) and Glycolysis Assay [Extracellular acidification] (ab197244) (black column) show the shift between mitochondrial respiration and glycolysis and the cellular control of energy (ATP; measured 1h post-treatment using Luminescent ATP Detection Assay kit (ab113849) (striped column)).
-
Typical Lifetime profile of Glycolysis Assay for adherent cells, treated with typical control compounds, including Oxamic acid recommended as a negative control. The effect of Glucose Oxidase as a positive signal control is illustrated schematically.
-
Excitation and Emission spectra of Glycolysis Assay. Left panel shows normalized excitation (Ex 340 – 410nm; Peak 360-380nm). Right panel shows emission maxima (Em 590, 615 and 690nm) fold increase between pH6.0 and pH7.5.
数据表及文件
-
SDS download
-
Datasheet download
文献 (26)
ab197244 被引用在 26 文献中.
- Lang L et al. Tumor suppressive role of microRNA-4731-5p in breast cancer through reduction of PAICS-induced FAK phosphorylation. Cell Death Discov 8:154 (2022). PubMed: 35379785
- Fang Y et al. LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis. Int J Biol Sci 18:3082-3101 (2022). PubMed: 35541892
- Zhang J et al. TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice. Front Cell Dev Biol 10:757042 (2022). PubMed: 35547804
- Horikawa M et al. Dual roles of AMAP1 in the transcriptional regulation and intracellular trafficking of carbonic anhydrase IX. Transl Oncol 15:101258 (2022). PubMed: 34742153
- Ma R et al. Dual Roles of Lactate in EGFR-TKI-Resistant Lung Cancer by Targeting GPR81 and MCT1. J Oncol 2022:3425841 (2022). PubMed: 36545125