重组Anti-CC2D1A抗体[EPR18421] (ab191472)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18421] to CC2D1A
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-CC2D1A抗体[EPR18421]
参阅全部 CC2D1A 一抗 -
描述
兔单克隆抗体[EPR18421] to CC2D1A -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: HeLa, HEK-293, Jurkat, MCF7, C6, Raw264.7, PC12, NIH 3T3 cell lysates. Mouse brain lysate. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18421 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab191472于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/250.
|
|
ICC/IF |
1/150.
|
|
WB |
1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 104 kDa).
|
说明 |
---|
Flow Cyt (Intra)
1/250. |
ICC/IF
1/150. |
WB
1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 104 kDa). |
靶标
-
相关性
CC2D1A belongs to the CC2D1 family. It contains one C2 domain. It is a transcription factor that binds specifically to the FRE (five repressor element) and represses HTR1A gene transcription in neuronal cells. The combination of calcium and ATP specifically inactivates the binding with FRE. CC2D1A may play a role in the altered regulation of HTR1A associated with anxiety and major depression. It also mediates HDAC-independent repression of HTR1A promoter in neuronal cell. There are three named isoforms. Defects in CC2D1A are the cause of non-syndromic mental retardation autosomal recessive type 3 (MRT3). Patients display severe mental retardation and psychomotor development delay in early childhood. Non-syndromic mental retardation patients do not manifest other clinical signs. -
细胞定位
Cytoplasm. Nucleus. -
数据库链接
- Entrez Gene: 54862 Human
- Entrez Gene: 212139 Mouse
- Entrez Gene: 288908 Rat
- Omim: 610055 Human
- SwissProt: Q6P1N0 Human
- SwissProt: Q8K1A6 Mouse
- SwissProt: Q66HA5 Rat
-
别名
- Akt kinase interacting protein 1 antibody
- Coiled coil and C2 domain containing 1A antibody
- Coiled coil and C2 domain containing protein 1A antibody
see all
图片
-
All lanes : Anti-CC2D1A antibody [EPR18421] (ab191472) at 1/20000 dilution
Lane 1 : Hela (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 104 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds5% NFDM/TBST: Blocking and diluting buffer.
-
All lanes : Anti-CC2D1A antibody [EPR18421] (ab191472) at 1/5000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 104 kDa
Observed band size: 130,67 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds5% NFDM/TBST: Blocking and diluting buffer.
The band at 67KD is an isoform of CC2D1A.
Rogaeva A and Albert PR. Eur J Neurosci. 2007; 26(4):965-974.
-
Anti-CC2D1A antibody [EPR18421] (ab191472) at 1/20000 dilution + Mouse brain tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 104 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds5% NFDM/TBST: Blocking and diluting buffer.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CC2D1A with ab191472 at 1/150 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasm staining on HeLa cell line.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
1. ab191472 at 1/150 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CC2D1A with ab191472 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (2)
ab191472 被引用在 2 文献中.
- Kondaurova EM et al. Genetic Background Underlying 5-HT1A Receptor Functioning Affects the Response to Fluoxetine. Int J Mol Sci 21:N/A (2020). PubMed: 33233644
- Schroeder M et al. Hypothalamic miR-219 regulates individual metabolic differences in response to diet-induced weight cycling. Mol Metab 9:176-186 (2018). PubMed: 29398616