Anti-ABCA1抗体[AB.H10] (ab18180)
Key features and details
- Mouse monoclonal [AB.H10] to ABCA1
- Suitable for: IHC-P, Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-ABCA1抗体[AB.H10]
参阅全部 ABCA1 一抗 -
描述
小鼠单克隆抗体[AB.H10] to ABCA1 -
宿主
Mouse -
经测试应用
适用于: IHC-P, Flow Cyt, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human ABCA1 aa 1800-2260.
Database link: O95477 -
阳性对照
- testis, liver, and brain tissue (negative control: muscle tissue)
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常规说明
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Protein A purified from TCS -
克隆
单克隆 -
克隆编号
AB.H10 -
同种型
IgG1 -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18180于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt | (3) |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB | (13) |
1/200 - 1/500. Predicted molecular weight: 254 kDa.
Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate. |
说明 |
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IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/200 - 1/500. Predicted molecular weight: 254 kDa. Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate. |
靶标
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功能
cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport. -
组织特异性
Widely expressed, but most abundant in macrophages. -
疾病相关
Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux. -
序列相似性
Belongs to the ABC transporter superfamily. ABCA family.
Contains 2 ABC transporter domains. -
结构域
Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. -
翻译后修饰
Phosphorylation on Ser-2054 regulates phospholipid efflux.
Palmitoylation by DHHC8 is essential for membrane localization. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 19 Human
- Entrez Gene: 11303 Mouse
- Omim: 600046 Human
- SwissProt: O95477 Human
- SwissProt: P41233 Mouse
- Unigene: 429294 Human
- Unigene: 277376 Mouse
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别名
- ABC 1 antibody
- ABC Transporter 1 antibody
- ABC-1 antibody
see all
图片
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Overlay histogram showing HepG2 cells stained with ab18180 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18180, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
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Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous bloting with anti-actin antibody was used for protein loading control. Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous blotting with anti-actin antibody was used for protein loading control.
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All lanes : Anti-ABCA1 antibody [AB.H10] (ab18180) at 1/1000 dilution
Lanes 1 & 3 & 5 : Wild type mouse liver tissue lysate
Lanes 2 & 4 & 6 : Knockout mouse liver tissue lysate
Lysates/proteins at 100 µg per lane.
Predicted band size: 254 kDa
Observed band size: 254 kDaThe lower bands are from the secondary anti-mouse antibody reacting to the endogenous mouse antibodies from the tissue.
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Immunoreactivity of pancreas tissue for ABCA1 protein. Left panel: strong immunopositivity of exocrine glandular cells of the pancreas, especially in the basal region (arrows). Interstitial microvascular cells are apparently negative. Cells within the islet were weakly positive (data not shown). Right panel: the negative control. Hematoxylin counterstain.
数据表及文件
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SDS download
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Datasheet download
文献 (188)
ab18180 被引用在 188 文献中.
- Lu X et al. MicroRNA-320b Modulates Cholesterol Efflux and Atherosclerosis. J Atheroscler Thromb 29:200-220 (2022). PubMed: 33536383
- Pushkarsky T et al. Abundance of Nef and p-Tau217 in Brains of Individuals Diagnosed with HIV-Associated Neurocognitive Disorders Correlate with Disease Severance. Mol Neurobiol 59:1088-1097 (2022). PubMed: 34843091
- Gui M et al. Modified Yuejuwan Inhibited Cholesterol Accumulation and Inflammation in THP-1 Macrophage-Derived Foam Cells by Inhibiting the Activity of the TRIM37/TRAF2/NF-κB Pathway. Evid Based Complement Alternat Med 2022:6400517 (2022). PubMed: 35310029
- Prijic S & Chang JT ABCA1 Expression Is Upregulated in an EMT in Breast Cancer Cell Lines via MYC-Mediated De-Repression of Its Proximal Ebox Element. Biomedicines 10:N/A (2022). PubMed: 35327383
- Gao J et al. Suppression of the ABCA1 Cholesterol Transporter Impairs the Growth and Migration of Epithelial Ovarian Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35454786